Fig. 7

Regulatory effects of Osteoking on the expression of key proteins for necroptosis in bone tissues of bone defect rats. A–C The expression levels of p-RIPK1, p-RIPK3, p-MLKL proteins in bone tissue of rats in different groups detected by immunohistochemical assay. Original magnifications, ×100 and ×400; scale bars, 200 μm and 50 μm. D–F Immunoreactive scores of p-RIPK1, p-RIPK3, p-MLKL proteins expression levels in bone tissues of rats in different groups. G–I The activity of key proteins for necroptosis in bone tissue supernatants were detected by ELISA. G Receptor-interacting serine/threonine-protein kinase 1 (RIPK1; U/L). H Receptor-interacting serine/threonine-protein kinase 3 (RIPK3; ng/mL). I Mixed-lineage kinase domain-like protein (MLKL; ng/mL). Bars show mean ± SD; ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 indicate the significance compared to the normal control group, respectively; *P < 0.05, **P < 0.01 and ***P < 0.001 indicate the significance compared to the model group, respectively