Fig. 6

SIRT1 mediates Nrf2-induced effects of liquiritigenin on myofibroblast differentiation. A–D The influence of liquiritigenin on SIRT1 expression in vitro was evaluated through qPCR analysis and Western blotting. Scale bars represent 100 μm. E–G The impact of liquiritigenin on SIRT1 expression in vivo was assessed through qPCR analysis and Western blotting. H–I The efficiency of SIRT1 siRNA was evaluated through qPCR analysis and Western blotting. (K-N) qPCR analysis revealed the effects of liquiritigenin treatment on myofibroblast collagen I, α-SMA, HO-1, and NQO-1 mRNA levels under control siRNA and SIRT1 siRNA conditions following TGF-β1 intervention. O–T Western blotting assessed the protein expression levels of collagen I, α-SMA, CAT, HO-1, and NQO-1 in myofibroblasts treated with liquiritigenin under control siRNA and SIRT1 siRNA conditions following TGF-β1 intervention. U Immunofluorescence microscopy displayed the effect of liquiritigenin treatment on collagen I and α-SMA expression levels in myofibroblasts under control siRNA and SIRT1 siRNA conditions following TGF-β1 intervention, with scale bars representing 100 μm. V H2DCFCDA probe indicated ROS levels. MitoSOX probe indicated mitochondrial ROS levels. Scale bars represent 100 μm. Data are presented as means ± standard deviation, and each experiment was independently repeated at least three times. (*P < 0.05, **P < 0.01, ***P < 0.001)