Fig. 7
From: The SIRT1/Nrf2 signaling pathway mediates the anti-pulmonary fibrosis effect of liquiritigenin
Liquiritigenin activated SIRT1 suppresses phosphorylation and acetylation of Smad. A Immunofluorescence microscopy displayed the effect of liquiritigenin treatment on Smad2/3 translocation in myofibroblasts with scale bars representing 100 μm. B Protein levels of Pho-Smad3 and Pho-Smad4 were assessed using Western blotting. C Deacetylation on Smad3 and Smad4 by liquiritigenin were measured by Western blotting. D Western blotting showed the interactions between Sirt1 and Smad3. Data are presented as means ± standard deviation, and all experiments were independently repeated at least three times. (**P < 0.01, ***P < 0.001)