Fig. 9

The SIRT1/Nrf2 signaling pathway mediates the effects of liquiritigenin on bleomycin-induced pulmonary oxidative stress in mice. A DHE staining reflects lung ROS levels. Scale bars represent 100 μm. B–D Biochemical quantification of MDA and GSH levels, as well as SOD activity. E, F qPCR analysis of HO-1 and NQO-1 mRNA levels. G–J Western blotting determined the protein expression levels of CAT, HO-1, and NQO-1. Data are presented as means ± standard deviation, and all experiments were independently repeated at least three times. (*P < 0.05, **P < 0.01, ***P < 0.001)