Fig. 1

GLP ameliorated LD-induced CG formation and metabolic disorders. A Representative gallbladders and gallstones. B Incidence of gallstone. C Grades of experimental CGs. D Concentrations of TC, BA and PL in gallbladder bile. E CSI of gallbladder bile. F Representative images of H&E and Oil Red O staining of liver tissue sections. Scale bars, 50 μm. G Percentage of Oil Red O positive area. H Hepatic TC and TG levels. I Serum TC and TG levels. Data are shown as the mean ± SEM (n = 10). *p < 0.05, **p < 0.01. ND: normal diet; LD: lithogenic diet; GLPL: low-dose Ganoderma lucidum polysaccharide; GLPH: high-dose Ganoderma lucidum polysaccharide; CG: cholesterol gallstone; TC: total cholesterol; BA: bile acid; PL: phospholipid; CSI: cholesterol saturation index; H&E: hematoxylin and eosin; TG: triglyceride