Fig. 2

GLP downregulated intestinal FXR-FGF15 and hepatic FXR-SHP signaling. A The mRNA expression of FXR and FGF15 in the ileum (n = 8). B The protein expression and quantification of FGF15 in the ileum (n = 6). C Serum FGF15 level (n = 10). D, E The mRNA expression of FGFR4, FXR and SHP in the liver (n = 8). F The protein expression and quantification of SHP in the liver (n = 6). The mRNA expression and protein expression were normalized to GAPDH and shown as fold changes relative to control group. Data are shown as the mean ± SEM. *p < 0.05, **p < 0.01. ND: normal diet; LD: lithogenic diet; GLPL: low-dose Ganoderma lucidum polysaccharide; GLPH, high-dose Ganoderma lucidum polysaccharide