Fig. 3

GLP promoted BA synthesis and inhibited cholesterol synthesis and efflux in liver. A The mRNA expression of CYP7A1, CYP8B1, CYP27A1 and CYP7B1 in the liver (n = 8). B The protein expression and quantification of CYP7A1 and CYP7B1 in the liver (n = 6). C, D The mRNA expression of BSEP, MRP2, SCAP and SREBP2 in the liver (n = 8). E The protein expression and quantification of SREBP2 in the liver (n = 6). F The mRNA expression of ABCG5 and ABCG8 in the liver (n = 8). G The protein expression and quantification of ABCG5 and ABCG8 in the liver (n = 6). The mRNA expression and protein expression were normalized to GAPDH and shown as fold changes relative to control group. Data are shown as the mean ± SEM. *p < 0.05, **p < 0.01. ND: normal diet; LD: lithogenic diet; GLPL: low-dose Ganoderma lucidum polysaccharide; GLPH: high-dose Ganoderma lucidum polysaccharide; BA: bile acid