Fig. 6

FXR activation reversed the protective effects of GLP in LD-fed mice. A Representative gallbladders and gallstones. B Incidence of gallstone. C Grades of experimental CGs (n = 10). D Concentrations of TC, BA and PL in gallbladder bile (n = 10). E CSI of gallbladder bile (n = 10). F Serum TC level (n = 10). G The mRNA expression of FGF15 in the ileum (n = 8). H The protein expression and quantification of FGF15 in the ileum (n = 6). I Serum FGF15 level (n = 10). J, K The mRNA expression of FGFR4 and SHP in the liver (n = 8). L The protein expression and quantification of SHP in the liver (n = 6). The mRNA expression and protein expression were normalized to GAPDH and shown as fold changes relative to control group. Data are shown as the mean ± SEM. *p < 0.05, **p < 0.01. LD: lithogenic diet; GLP: Ganoderma lucidum polysaccharide; CG: cholesterol gallstone; TC: total cholesterol; BA: bile acid; PL: phospholipid; CSI: cholesterol saturation index