Table 1 Primers for qPCR
Gene | Primer sequence (5′ to 3′) | |
|---|---|---|
FXR | Forward | GGGATGAGCTGTGTGTTGTCTGTG |
| Â | Reverse | GTCTGTTGGTCTGCCGTGAGTTC |
FGF15 | Forward | CGGCAAGATATACGGGCTGATTCG |
| Â | Reverse | TCTACATCCTCCACCATCCTGAACG |
FGFR4 | Forward | GGTGACCGAGGATGATGTGATGAAG |
| Â | Reverse | AGAGACAGCCAGCAGGACCTTG |
SHP | Forward | CAGGTCGTCCGACTATTCTGTATGC |
| Â | Reverse | TACCGCTGCTGGCTTCCTCTAG |
CYP7A1 | Forward | GTATGCCTTCTGCTACCGAGTGATG |
| Â | Reverse | GTGCGTCTTAGCCTTCTCCATGTC |
CYP8B1 | Forward | TGGACAGCGTGATGGAGGAGAG |
| Â | Reverse | GGTGGATCTTCTTGCCCGACTTG |
CYP27A1 | Forward | AAGGACCACCGAGACCACAAGG |
| Â | Reverse | GGAGGGTTTCAGGCAGCCAATC |
CYP7B1 | Forward | TTGGCTTCCTTATCTTGGCATGGC |
| Â | Reverse | TCGCTGATAATCGGCTGCTGAAC |
BSEP | Forward | GAGTTGGTGTGGCTGTCCTTATCC |
| Â | Reverse | ATCTGGTCGGCAATGGCTTCATC |
MRP2 | Forward | CCTATTCCTGCCTGTTCTTCGTCTC |
| Â | Reverse | TCTGCTTCTTGGTCAATCCGTGTG |
SCAP | Forward | TACAGCAGCAGCAACACAGTGAC |
| Â | Reverse | ACAGGAAGCGGTACAGGTGAGG |
SREBP2 | Forward | CAAAGAAGGAGAGAGGCGGACAAC |
| Â | Reverse | GGCGGAGACATCAGAAGGACATTC |
ABCG5 | Forward | CATCGCAGCCCAACTCCTTCAG |
| Â | Reverse | CCACAGAACACCAACTCTCCGTAAG |
ABCG8 | Forward | GCTCGTGTGGTTGGTGGTCTTC |
| Â | Reverse | GTTGCCGATTTGTGTGGTGTAAAGG |
GAPDH | Forward | AGG​TCG​GTG​TGA​ACG​GAT​TTG |
|  | Reverse | TGT​AGA​CCA​TGT​AGT​TGA​GGT​CA |