Fig. 1

Effect of GW on Dexa-induced myotube atrophy. A Differentiated C2C12 cells were fixed and stained with α-actinin antibody (scale bar, 200 μm) (left). Myotube diameter was calculated as the average diameter of α-actinin-positive multinucleated myotubes (right). B The expression levels of Atrogin-1 and MuRF1 quantified by qRT-PCR in Dexa-treated myotubes. C The protein levels of total MHC, MHCI, IIa, IIb, Atrogin-1, MuRF1, and Vinculin measured by western blot in Dexa-treated C2C12 cells. Results are expressed as mean ± SD. One-way ANOVA was used to compare more than two groups, followed by Bonferroni post-hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001 versus the Dexa-treated myotubes