Fig. 4

Effect of GW on muscle atrophy and protein synthesis-related factors. The protein levels of A total MHC, I, IIa, IIb and B Atrogin-1, MuRF and C p-FoxO3, FoxO3 in quad muscles were determined by western blot. D The cytosolic and nuclear expressions of GR and FoxO3a were measured. E The expression of p-Akt, Akt, p-mTOR, mTOR, p-S6K, S6K, p-4EBP1, 4EBP1, and Vinculin in quad muscle as determined by western blot