Fig. 2

TRQ attenuates the release of NO, IL6 and TNFα in DENV-2 infected murine microglial BV2 cells. A The cytotoxicity of TRQ in BV2 cells. BV2 cells were incubated with TRQ for 24 h and cell viability was determined by MTT assay. B–D TRQ inhibits the release of NO, IL6 and TNFα in BV2 cells. After exposure to DENV-2 (0.5 MOI) in the presence or absence of TRQ for 24 h, the secretion of NO (B), IL6 (C) and TNFα (D) was measured by NO assay kits or ELISA kits. ^*P < 0.05, ^***P < 0.001 vs. control group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. DENV-2 group