Fig. 6

TRQ inhibits the activation of NLRP3 in brain tissues of DENV-infected mice. A, B TRQ decreases the mRNA expression levels of Nlrp3 (A) and Il1b (B) in DENV-infected mice (n = 6). The brain tissues of the mice were harvested and total mRNAs were extracted and analyzed by qRT‒PCR. C–E TRQ decreases the protein expression level of CASP1 in DENV-infected mice. The brain tissues of the mice were harvested and total protein lysates were collected and analyzed by Western blotting. F TRQ decreases the release of IL1B in DENV-infected mice. The level of IL1B in the brain was measured by ELISA (n = 6). G TRQ inhibits NLRP3 in DENV-infected mice. The brain tissues were sectioned and the expression of NLRP3 in the mouse brain tissues was determined with anti-NLRP3 antibodies by immunofluorescence staining (200 ×). DAPI was used to stain the nucleus. ^**P < 0.01, ^***P < 0.001 vs. control group, ^#P < 0.05, ^##P < 0.01 vs. DENV group. (F) The mechanism of TRQ on dengue encephalitis. The schematic was drawn with BioRender (https://biorender.com/)