Fig. 6

Effect of HPM treatment on T lymphocytes in the spleens of immunosuppressed rats. A CD4+, CD8+ cells were gated by the protocol. B Flow cytometric identification of CD4 + , CD8 + cells in the spleen. C, D Absolute count statistics of CD4+, CD8+ cells between different groups in spleen tissue (n = 5 per each group). Data were analyzed using Kruskal–Wallis H-statistic test. **p < 0.01, ***p < 0.001vs NG; ^#p < 0.05 vs CTX. NG: normal group; CTX: cyclophosphamide; HPM: Herb-partitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole