Fig. 7

Results of the CD28 mRNA level and correlation between CD28 and B7-1 in spleen of immunosuppressed rats (n = 3–5 per each group). A immunofluorescence results, a–f Pearson correlation scatterplot; B CD28 IOD results; C CD28 mRNA level; D CD28 and B7-1 colocalization results. Data are expressed as means ± SD, One-way ANOVA tests with LSD-t post-hoc tests executed and Pearson correlation coefficient, Kruskal–Wallis one-way ANOVA test. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs NG; ^##p < 0.01, ^###p < 0.001 vs CTX; ^%p < 0.05, ^%%%p < 0.001 vs HPM. IOD: integrated optical density; SD: standard deviation; ANOVA: analysis of variance; LSD-t: Least Significant Difference-t, NG: normal group; CTX: cyclophosphamide; HPM: Herb-partitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole