Table 1 Primer sequences
Gene | Forward primer | Reverse primer |
|---|---|---|
CD28 | 5′GGGTGGAGGTAGGTTGGAGTA3′ | 5′AGGAAGTGAGGAAACAAGCCC3′ |
CTLA-4 | 5′CTTGCTGCAGTTAGTTCGGG3′ | 5′GGCTCTGTTGGGGGCATTTT3′ |
B7-1 | 5′AAGGGAAGTTGGAAAGGGGAAA3′ | 5′GAACCGAGGTCTTGAGCCTT3′ |
GAPDH | 5′ACTCTACCCACGGCAAGTTC3′ | 5′TGGGTTTCCCGTTGATGACC3′ |