Fig. 7

SKP inhibited the HIF-1α/HO-1 Signaling Pathway. A–D Western blot for HIF-1α and HO-1 in kidney tissues of each group and the quantitative analysis for Western blotting results of HIF-1α and HO-1. E, F The mRNA levels of HIF-1α and HO-1 in the renal tissues of each group. G Representative pictures of ROS changes in mice kidneys (bar = 50 µm). H Fluorescence intensity of ROS by image J. I–K Immunohistochemical staining assays of HIF-1α and HO-1 in kidneys and the quantifications of the expressions. (400 × magnification, bar = 100 µm). L Prussian blue staining of kidney tissues of mice. (800× magnification, bar = 50 µm). M Ferrous ion content in mice kidney tissues. *P < 0.05, **P < 0.01, and ***P < 0.001vs. control group. ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 vs. DKD group, ns no significance