Fig. 2

The combination of PTX and CK can regulate the balance of Treg and Th17. A, B The Lewis cells cocultured with SPC were treated with 60 μM CK combined with 10 nM PTX for 48 h, apoptotic cells (%) and non-apoptotic death cells were detected by Flow Cytometry. C, D The Lewis cells cultured alone or cocultured with SPC were treated with 60 μM CK, 10 nM PTX, or both for 48 h, the expression of pyroptosis-related proteins was detected by Western blot, β-actin was used as loading control. E, F The Lewis cells were cocultured with SPC and treated with 60 μM CK, 10 nM PTX, or both for 48 h. CD4⁺CD25⁺ cells and CD4⁺CD196⁺ cells in CD4⁺SPC were determined by Flow Cytometry, and the ratio of CD4⁺CD25⁺ cells to CD4⁺CD25⁺ cells was calculated. G, H The Lewis cells were cocultured with SPC and treated with 60 μM CK, 10 nM PTX, or both for 48 h. Foxp3⁺ cells and RORγt⁺ cells in SPC were detected by Flow Cytometry, and the ratio of Foxp3⁺ cells to RORγt⁺ cells was calculated. I The Lewis cells were cocultured with SPC and treated with 60 μM CK, 10 nM PTX, or both for 48 h. The concentration of IL-10 and IL-17A in co-culture system was determined by ELISA. J The Lewis cells were cocultured with SPC and 1X protein transport inhibitor cocktail and treated with 60 μM CK, 10 nM PTX, or both for 48 h. the IL-17A⁺ cells or IL-10⁺ cells in CD3⁺CD4⁺ cell population were determined by Flow Cytometry. K The relative Geomean of IL-17A or IL-10 to ctrl group. L, M The Lewis cells were cocultured with SPC and treated with 60 μM CK, 10 nM PTX, or both for 48 h. CD3⁺CD4⁺ cells and CD3⁺CD8⁺ cells in SPC were determined by Flow Cytometry, and the ratio of CD3⁺CD4⁺ cells to CD3⁺CD8⁺ cells was calculated. N The Lewis cells were cocultured with SPC and treated with 60 μM CK, 10 nM PTX, or both for 48 h. The concentration of IFN-γ in co-culture system was determined by ELISA. Data are mean ± SD for ≥ 3 independent experiments; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, vs Ctrl