Fig. 5

The combination of PTX and CK can induce pyroptosis of Lewis cells in the co-culture system. A Lewis cells cultured alone or in the co-culture system were treated with CK or PTX or both. The expression of Granzyme B and Perforin 1 in Lewis cells was detected by Western blot, β-actin was used as loading control. B–D Lewis cells cultured alone or in the co-culture system were pretreated with 20 μM Z-VAD-FMK for 1 h, and then treated with 60 μM CK, 10 nM PTX, or both for 48 h. Apoptotic cells (%) and non-apoptotic death cells (%) were detected by Flow Cytometry. E, F Lewis cells cultured alone or in the co-culture system were pretreated with 20 mM d-mannose for 3 h, and then treated with 60 μM CK, 10 nM PTX, or both for 48 h. Pyroptosis (%) was detected by Flow Cytometry. Data are mean ± SD for ≥ 3 independent experiments; P values have no significant difference, vs Ctrl