Fig. 6

The combination of PTX and CK can inhibit the growth of transplanted tumor in mice by activating immune function. A–C Average tumor volume (A, B) and Average tumor weight (H) in Ctrl, CK, PTX and PTX + CK group. D, E The expression of Granzyme B, Perforin 1, Caspase-3, Cle-Caspase3, GSDME and GSDME-N in tumor cells from every group was determined by Western blot, β-actin was used as loading control. F The immunohistochemical analysis performed with anti-Ki67, anti-Cle-Caspase3 and anti-GSDME-N in tumor tissue, Scale bar: 50 μm. G The spleen cells were extracted from every group, CD4⁺CD25⁺ cells and CD4⁺CD196⁺ cells in SPC were determined by Flow Cytometry. H The spleen cells were extracted from Blank group, Ctrl group and PTX + CK group, Foxp3⁺ cells and RORγt⁺ cells in SPC were detected by Flow Cytometry. I, J The tumor infiltrating lymphocytes were extracted from every group, the RORγt⁺IL17A⁺ cells or CD25⁺Foxp3⁺ cells in CD45⁺CD4⁺ cell population were determined by Flow Cytometry. K–M The tumor infiltrating lymphocytes were extracted from every group, the CD4⁺ cells or CD8⁺ cells were determined by Flow Cytometry. N The immunohistochemical analysis performed with anti-p-STAT3 and anti-p-STAT5 in spleen tissue, Scale bar: 100 μm. O The concentration of IFN-γ in the blood samples from each group was determined by ELISA. Data are mean ± SD for ≥ 3 independent experiments; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, vs Ctrl