Fig. 5

The Bufei Yishen formula (BYF: 25, 50 μg/ml) reduces paracellular permeability and increases the expression of epithelial junction in CSE-induced BEAS-2B cells. A, B TEER and permeability of BEAS-2B cell monolayers. C–I The levels of ZO-1, ZO-2, occludin, E-cadherin, p-ERK/1/2, and p-EGFR proteins in BEAS-2B cells. J The levels of ZO-1 and occludin proteins in BEAS-2B cells were detected by immunofluorescence. Data are expressed as the mean ± SEM (n = 3), *P < 0.05, **P < 0.01, versus the Control group; ^#P < 0.05, ^##P < 0.01, versus the Model group