Fig. 4

ELG protects against acute ethanol (ETOH)—induced liver enzymes dysfunction and impairment of hepatocyte viability in mice and cell models. A, B Serum ALT and AST levels. n = 7. C–E Mouse representative western blot images and relative protein levels in the liver. n = 5. ^#p < 0.05, AALI vs. control group; *p < 0.05, AALI vs AALI + ELG group. F, G CCK8 evaluation in AML-12 cells receiving 24-h DMSO, ELG (0, 10, 25, 50, 100, 200 and 400 µg.mL⁻¹), or ethanol (0, 62.5, 125, 250 and 500 mM) treatment. n = 3. ^#p < 0.05, control vs ELG or ethanol group. AML-12 cells were incubated with DMSO and ELG (0, 25, 50 and 100 µg.mL⁻¹) with or without ethanol (500 mM) for 24 h. H CCK8 assay. n = 3. I, J Cell culture supernatants ALT and AST levels. n = 4. (K) The relative mRNA level of Casp3 in AML-12 cells. n = 3. L, M Representative western blot image and quantitative data of protein expression of cle-Casp3 in AML-12 cells. n = 3. Experimental data were statistically analysed as the means ± SEM. ^#p < 0.05, ethanol vs. DMSO control group; *p < 0.05, ethanol vs ethanol + ELG group