Fig. 5

ELG attenuates oxidative stress in liver and AML-12 cells under ethanol challenge. A, B Analysis of hepatic MDA and GSH contents. n = 6. C–F mRNA determination of hepatic oxidative stress markers. n = 5. ^#p < 0.05, AALI vs. control group; *p < 0.05, AALI vs AALI + ELG group, NS not significance. G, H The representative fluorescent images and relative fluorescent intensity of ROS staining in AML-12 cells receiving 24-h co-incubation of ELG (100 µg.mL⁻¹) and ethanol (500 mM). Original magnification, × 200; Scale bars, 100 µm. n = 3. All experimental data were statistically analysed as means ± SEM. ^#p < 0.05, ethanol vs. DMSO control group or *p < 0.05, ethanol vs ethanol + ELG group