Fig. 6

ELG actives hepatic AMPK-NLRP3 signaling in acute binge alcohol-fed mice. Representative western blot image (A) and relative protein expression of p-Ampk/Ampk (B), Nlrp3/β-Actin (C), and cle-Casp1/β-Actin (D) in the liver of mice from control, AALI and AALI + ELG group. n = 5, per group. E Hepatic immunohistochemical analysis of Nlrp3. n = 5, per group. Original magnification, × 100; Scale bars, 200 µm. F–I Relative mRNA levels of FAO-involved hepatic AMPK downstream genes. n = 5. Statistical analysis of all experimental data was performed as means ± SEM. ^#p < 0.05, AALI vs. control group; *p < 0.05, AALI vs AALI + ELG group, NS not significance