Fig. 7

ELG regulates AMPK-NLRP3 signaling in mouse hepatocytes. AML-12 cells were treated by DMSO and ELG (0, 50 and 100 µg.mL⁻¹) with or without ethanol (500 mM) for 24 h. Representative western blot image (A) and relative protein levels of genes (B–D) in AMPK-NlRP3 signaling. n = 3, per group. E, F AML-12 cells receiving culture of DMSO, ethanol (500 mM) or LPS (100 ng.mL⁻¹) and treated in the presence or absence of ELG (100 µg.mL⁻¹) were used for subsequent analysis of relative mRNA levels of Il-1β. n = 3, per group. G Cell supernatant Il-1β concentration in AML-12 with ethanol (500 mM) stimulation and ELG (100 µg.mL⁻¹) treatment. n = 3, per group. Data were statistically analysed as means ± SEM. ^#p < 0.05, as compared with control group; *p < 0.05, ELG treatment vs ethanol or LPS group