Fig. 8

AMPK inactivation blunts the inhibitory effects of ELG on NLRP3 signaling and impairment in mouse hepatocytes. AML-12 cells insulted by ethanol (500 mM) were treated by DMSO and ELG (100 µg.mL⁻¹) with or without CC (10 µM) for 24 h. A Cell viability assessment. n = 3. B, C ALT and AST levels in cell culture supernatants. n = 3. Representative western blot image (D) and relative protein expression of Nlrp3 (E), cle-Casp1 (F), Chop (G) and p-Jnk/Jnk (H) were displayed. n = 3. I Determination of relative mRNA expression of Il-1β. n = 3. J The cell supernatant Il-1β level. n = 4, per group. Data were statistically analysed as means ± SEM. *p < 0.05, as compared with ethanol group or ethanol + ELG + CC group, NS not significance