Fig. 2

As-IV, Ta-IIA, and Co inhibited myocardial cell apoptosis, oxidative stress, and inflammatory response in MIRI. A Representative images of apoptotic cardiomyocytes. The apoptotic cells were detected by TUNEL (green), and the nuclei were detected by DAPI (blue). Scale bar = 50 μm. B Representative blots of the apoptosis-related proteins Bax, Bcl2, Caspase3, and Cleaved Caspase3 in myocardial tissue, as well as quantitative analysis of Bax, Bcl2, and Cleaved Caspase3. C Representative images of ROS content in cardiomyocytes. ROS was detected by DHE fluorescence staining (red), and nuclei were detected by DAPI (blue). scale bar = 20 μm. D Myocardial glutathione (GSH) activity, malondialdehyde(MDA) content, superoxide dismutase (SOD) activity. E. The mRNA quantification of cytokines IL-6, IL-1β, TNFα, iNOS in cardiomyocardial tissues. Data are presented as mean ± SEM. (n = 6 in each group). *p < 0.05, **p < 0.01, ***p < 0.001