Fig. 3

Effects of As-IV and Ta-IIA on HR-induced apoptosis, oxidative stress, and inflammation in HL1 cells. A As-IV and Ta-IIA treatment dose- dependently increased the viability of HR- injured HL1 cells. B The increase in cell activity with Co treatment was greater in HR-injured HL1 cells than in those treated with Ta-IIA and As-IV. Ta-IIA was 1 μm, and As-IV was 50 μm. Co was a combination of Ta-IIA1 μm and As-IV 50 μm. C. Representative results of apoptosis rates measured in HL1 cells using flow cytometry. D Quantitative analysis of the apoptosis rate in (C). E Representative western blots of Bax, Bcl2, Cleaved Caspase3. F. The quantitative analysis of protein expression in (E). G Representative picture of the fluorescence curve of DCF in HL1 cells and the quantitative analysis of DCF mean fluorescence intensity. This was detected by flow cytometry. H. Cellular MDA content, GSH activity, SOD activity. I. Quantitative analysis of cytokines IL-6, IL-1β, TNFα and iNOS mRNA in HL1 cells. Data are presented as mean ± SEM. (n = 3 in each group). *p < 0.05, **p < 0.01, ***p < 0.001, ns = not statistically significant