Fig. 4

As-IV and Ta-IIA inhibited the STING pathway. A Chemical structure and representative simulations of the binding to the STING protein for As-IV and Ta-IIA. B Representative western blots and quantification of STING pathway-associated proteins in myocardial tissue. C Quantification of mRNA expression of the cytokines Ifi44, Cxcl10 and Ifnb1 downstream of the STING pathway caused by MIRI. D Representative immunofluorescence staining of p-STING in HL1 cells. The p-STING proteins were stained red, and the nuclei were detected by DAPI (blue). Scale bar = 20 μm. E Representative western blots of STING pathway-associated proteins in HR-induced HL1 cells. F The quantitative analysis of protein expression in (E). Data are presented as mean ± SEM. (n = 6 in each group in vivo; n = 3 in each group in vitro). *p < 0.05, **p < 0.01, ***p < 0.001, ns = not statistically significant