Fig. 5

STING agonist treatment attenuated the myocardial protection of Ta-IIA, As-IV, and Co on MIRI mice. Ta-IIA treatment (10 mg/kg/d, i.p.), As-IV treatment (15 mg/kg/day, i.p.), Co (Ta-IIA 10 mg/kg/day + As-IV 15 mg/kg/day i.p.) treatment were given 7 days before reperfusion until tested after anesthesia. DiabZI (3 mg/kg, i.v.) was administered once every other day for a total of three times following reperfusion surgery. A Experimental procedures for As-IV, Ta-IIA and Co in mice after the addition of agonists. B Serum CK, CKMB levels, and serum LDH levels. C Representative digital images of heart sections by Evans blue and TTC double staining, and the percentage of infarct area. The blue-stained portion indicates the normal region, the red-stained portion indicates the ischemic region, and the white portion indicates the infarcted region. The ratio of the infarct area of the largest heart section to the total area of the section was chosen to be the percentage of infarct area. D Representative M-mode echocardiographic images, and the quantitative analysis of the LVEF and LVFS. E Representative picture of HE staining of myocardial tissue. Data are presented as mean ± SEM. (n = 6 in each group). *p < 0.05, **p < 0.01, ***p < 0.001