Fig. 4

CAC extract inhibited neutrophils extracellular traps (NETs) formation. A Representative image of purified neutrophils after Wright-Giemsa’s staining (scale bar, 100 μm). B CAC extract inhibited PMA-induced cfDNA release in neutrophils. C–E Representative immunofluorescence image of cfDNA (C), MPO (D) and CitH3 (E) staining of neutrophils (scale bar, 100 μm). PMA-induced (1 μM) cfDNA release in neutrophils was inhibited by CAC extract (50, 100, 200 μg/ml). The expressions of MPO and CitH3 induced by PMA (1 μM) in neutrophils were suppressed by CAC extract (50, 100, 200 μg/ml). *P < 0.05 vs. PMA group; **P < 0.01 vs. PMA group; ***P < 0.001 vs. PMA group; ^#P < 0.05 vs. control group