Fig. 1

CPT inhibits cell proliferation and cell cycle of melanoma cells. A MTT assay for detecting the effect of CPT on the viability of B16F10 cells. B EDU staining detection of the effect of CPT on the proliferation of B16F10 cells and analysis by flow cytometry. C Fluorescence results of EDU staining detection of the effect of CPT on the proliferation of B16F10 cells. D Detection of the effect of CPT on cell cycle using propidium iodide (PI) staining and analysis by flow cytometry. E The effect of CPT on cell apoptosis. FITC labeled Annexin V was used to detect early apoptotic cells, while PI labeled necrotic cells or cells that lost cell membrane integrity in the late stage of apoptosis. Flow cytometry was used to analyze the cells. Compared with the control, * P < 0.05, ** P < 0.01, *** P < 0.001