Fig. 5

AMPK mediates CPT inhibition of glycolysis in melanoma cells. A Expression of PRKAA2 gene in different tumors in GEPIA database (^*** P < 0.001). B Western blot was used to detect the effect of CPT on the expression of AMPK, p-AMPK and HIF-1α in B16F10 melanoma cells. C Quantitative results of AMPK, p-AMPK, HIF-1α. D Effect of CPT on p-AMPK expression in melanoma cells detected by cellular immunofluorescence. The effect of CPT combined with AMPK inhibitor compound C on B16F10 cells: E the morphology of melanoma cells under inverted microscopy. F MTT detection of the viability of melanoma cells. G, H Detection of apoptosis of melanoma cells by Flow cytometry. I, J The glucose consumption and lactate production. Compared with the control, * P < 0.05, ** P < 0.01, *** P < 0.001