Fig. 6

Knockdowning AMPK partially reverses CPT’s effect on melanoma cells in vitro. Si-AMPK mimics were used to knockdown the AMPK expression in A375 cells, then the cells were treated with CPT for 24 h followed with the following assays. A Western blot for the expression of AMPK in A375 cells. B CCK assay for detecting the effect of CPT on the viability of A375 cells. C Representative images and quantitative analysis was conducted on the formation of cell clones by colony-formation assay. D Fluorescence results of EDU staining on the proliferation of A375 cells. E Detection of glycolytic metabolites of A375 cells. F, G Western blot for HIF-1α and PFKFB3 and semiquantitative grayscale analysis. Compared with 10 μM CPT, * P < 0.05, ** P < 0.01, *** P < 0.001