Fig. 6

SalC attenuates cerebral ischemic stroke in tMCAO mice by inhibiting neuroinflammation via the TLR4-TREM1-NF-κB pathway. A The gene expressions of Trem1, Il6, and Cxcl1 detected by qRT-PCR to validate transcriptome sequencing results. β-actin was used as an internal reference. B The protein expression levels of TREM1, TLR4, p-p65, and p65 in brain tissue were analyzed by western blot. β-actin was used as an internal standard. C Quantification was performed by Image Lab software. Data are expressed as mean ± SEM, and differences between groups were determined by t-test, n = 4. ^###P < 0.001, ^#P < 0.05 versus the sham group; *P < 0.05, versus the model group