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Fig.3 | Chinese Medicine

Fig.3

From: Electroacupuncture pretreatment mediates sympathetic nerves to alleviate myocardial ischemia–reperfusion injury via CRH neurons in the paraventricular nucleus of the hypothalamus

Fig.3

Results of immunofluorescence staining in the PVN. A Location of PVN (magnification, × 5; scale bar, 200 µm). B Comparison of c-Fos-positive neuronal expression in the PVN of rats in each group (magnification, × 20; c-Fos, red; DAPI, blue; scale bar, 50 µm). C The statistical analysis of the number of c-Fos + neurons in the PVN in each group of rats. Data are expressed as the mean ± SD. ***p < 0.001, n = 6 rats/group. D Comparison of CRH neurons and GABA neurons co-labeled with c-Fos in the PVN of MIRI rats (magnification, × 40; scale bar, 20 µm). E The statistical analysis of the number of CRH neurons and GABA neurons co-labeled with c-Fos in the PVN of MIRI rats. Data are expressed as the mean ± SD. ***p < 0.001, n = 6 rats/group

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