Fig. 3

Juglone induced the ferroptosis in GBM cells. A, B The fluorescence dye DCFDA has been used to measure the ROS level in LN229 and T98G cells and quantitatively analyze the fluorescence intensity. C, D The expression of key proteins in the ferroptosis pathway in LN229 and T98G cells under the action of juglone through WB experiments. E LN229 and T98G cells with or without Fer-1 and observed for about 14 days under different juglone concentrations for cell clone formation. F Mitochondrial morphology in different groups was observed using transmission electron microscopy in T98G cells. Yellow arrows indicated normal mitochondrial morphology, and red indicated damaged mitochondrial morphology after juglone induction