Fig. 5

Juglone activates p38MAPK and regulates the Nrf2/GPX4 axis in GBM cells. A WB experimental analysis of the expression levels of p-p38, Nrf2, and GPX4 in LN229 and T98G cells incubated for 24 h with different concentrations of juglone combined or not combined with SB203580 (10 µM, preprocessed for 1 h). B, C Images and statistical analysis results of plate clone formation of LN229 and T98G cells incubated for about 14 days with different concentrations of juglone combined or not combined with SB203580 (10 µM, preprocessed for 1 h). D, E Determination of MDA and GSH content in LN229 and T98G cells after 24 h of action with different concentrations of juglone combined or not combined with SB203580 (10 µM, preprocessed for 1 h). F, G Determination of ROS levels in LN229 and T98G cells after 24 h of action with different concentrations of juglone combined or not combined with SB203580 (10 µM, preprocessed for 1 h) based on flow cytometry analysis. H. Protein expression levels of GPX4 and Nrf2 in LN229 and T98G cells after 24 h of treatment with juglone in combination with t-BHQ (20 µM, preprocessed for 1 h) or overexpression of Nrf2, based on WB experiment. I, J Status of cell clone formation and statistical analysis under the action of juglone combined or not combined with t-BHQ (20 µM, preprocessed for 1 h) for about 14 days. K, L Determination of MDA and GSH content in LN229 and T98G cells after 24 h of treatment with juglone in combination with t-BHQ (20 µM, preprocessed for 1 h) or overexpression of Nrf2. M, N Detection and statistical analysis of ROS levels in LN229 and T98G cells after 24 h of treatment with juglone in combination with t-BHQ (20 µM, preprocessed for 1 h) or overexpression of Nrf2 by flow cytometry analysis. *p < 0.05; **p < 0.01; ***p < 0.001; ns, no statistical significance