Fig. 10

IGLR contributes to the wound healing of diabetic wounds through a paracrine mechanism. A Masson trichrome staining for wound skin tissues 14 days postoperatively in diabetic mice (scale bar, 1000 μm, 50 μm). B–D α-SMA, TGF-β and COL1A1 mRNA levels within wound tissues in diabetic mice detected through qRT-PCR 14 days later (n = 6). E The effect of α-SMA and COL1A1 levels on collagen production in the db/db mice wound model after 14 days of drug administration detected by Western blot assay. F, G The quantitative results of E. H Immunofluorescence analysis on TGF-β (red) and α-SMA (green) within wound tissues in diabetic mice after 14-day IGLR treatment at varying doses (scale bar, 50 μm). I, J Quantification of TGF-β and α-SMA during immunofluorescence analysis (n = 3), ^###P < 0.005, ^####P < 0.001 versus positive control group, *P < 0.05, **P < 0.01, ***P < 0.005 versus vehicle group, one-way ANOVA and Tukey’s multiple comparison test. Results are shown to be means ± SEM