Fig. 2

IGLR scavenges oxygen free radicals. A The scavenging rate of ABTS⁺ and DPPH by IGLR. B–D Roles of IGLR (0, 50, 100 and 200 μg/mL) in SOD, CAT and MDA levels in macrophages by LPS (1 μg/mL) induced for 48 h. E IGLR effects on ROS in macrophages cells under 48-h LPS (1 μg/mL) stimulation. F Quantification of NRF2 and HO-1 during immunofluorescence analysis (n = 3). ^###P < 0.005,^####P < 0.001 versus positive control group, *P < 0.05,**P < 0.01, ***P < 0.005 versus LPS (1 μg/mL) stimulated group, one-way ANOVA and Tukey’s multiple comparison test. In addition, results are shown to be means ± SEM