Fig. 3

IGLR stimulates macrophage resistance to oxidative stress via NRF2/KEAP1 pathway. A–D NRF2, KEAP1, HO-1 and NQO1 mRNA levels by real-time PCR in LPS stimulated macrophages with IGLR treatment. E The western blots of NRF2, KEAP1, HO-1 and NQO1in LPS induced macrophage with 48-h IGLR (0, 50, 100 and 200 μg/ mL) treatment. F–I Quantitative results of (E) (n = 3). J Immunofluorescence analysis on HO-1 (red) and NRF2 (green) within macrophages with different concentrations of IGLR (scale bar, 50 μm). K, L Quantification of NRF2 and HO-1 levels during immunofluorescence analysis (n = 3). ^#P < 0.05, ^##P < 0.01, ^###P < 0.005 versus positive control group, *P < 0.05, **P < 0.01, ***P < 0.005 versus LPS (1 μg/mL) group, one-way ANOVA and Tukey’s multiple comparison test. In addition, results are shown to be means ± SEM