Fig. 4

IGLR enhances macrophage anti-inflammation through COX2/PGE2 signaling pathway. A, B Functions of IGLR (0, 50, 100 and 200 μg/mL) in PGE2 and IL-6 levels in LPS induced macrophages for 48 h. C–H COX2, IL-1β, IL-6, NLRP3, ASC and caspase1 mRNA expression with real-time PCR in LPS induced macrophages. I The western blots of COX2, NLRP3, NF-κB, IL-1β, and ASC within LPS could induce macrophages under 48-h IGLR (0, 50, 100 and 200 μg/ mL) treatment. J–N Quantitative results for (I) (n = 3). ^#P < 0.05, ^##P < 0.01, ^###P < 0.005, ^####P < 0.001 versus control group, *P < 0.05, **P < 0.01, ***P < 0.005 versus LPS (1 μg/mL) group, one-way ANOVA and Tukey’s multiple comparison test. Results are shown to be means ± SEM