Fig. 5

IGLR conditioned medium (IGLR-CM) improves the proliferation and migration of fibroblasts. A Calcein (green) and PI (red) staining for proliferation in L929 cells by 24-h IGLR-CM incubation at diverse concentrations (scale bar, 100 μm). B Cell transfer after 24 h incubation of fibroblasts with different concentrations of IGLR-CM (scale bar, 50 μm). C–E α-SMA, TGF-β and COL1A1 mRNA expression analyzed via real-time PCR in fibroblasts at 24 h of IGLR-CM intervention. F COL1A1 and α-SMA protein expression within L929 cells incubated with different concentrations of IGLR-CM. G, H Quantification of western blots (n = 3). I Immunofluorescence analysis on COL1A1 (red) and α-SMA (green) within L929 cells with IGLR-CM (scale bar, 50 μm). J, K Quantification of COL1A1 and α-SMA during immunofluorescence analysis (n = 3). ^##P < 0.01, ^###P < 0.005, ^####P < 0.001 versus control group, *P < 0.05, **P < 0.01, ***P < 0.005 versus LPS (1 μg/mL) group, one-way ANOVA and Tukey’s multiple comparison test. Results are shown to be means ± SEM