Fig. 7

IGLR accelerates diabetic wound repair via NRF2/Keap1 pathway. A–C SOD, CAT, and MDA levels within plasma after gavage of IGLR at varying concentrations for 7 and 14 days (n = 6). D–G The NRF2, KEAP1, HO-1 and NQO1 mRNA levels within wound tissues detected by qRT-PCR 7 and 14 days later (n = 6). H NRF2, KEAP1, HO-1 and NQO1 expression in wound tissue of diabetic mice was detected by Western blot at Days 14 with or without IGLR treatment. I–L The quantitative results of (H) (n = 3). (M) Immunofluorescence analysis on HO-1 (red) and NRF2 (green) within wound tissues of diverse groups (scale bar, 50 μm). N, O Quantification of NRF2 and HO-1 in immunofluorescence staining (n = 3). ^##P < 0.01, ^####P < 0.001 versus positive control group, *P < 0.05, **P < 0.01, ***P < 0.005 versus vehicle group, one-way ANOVA and Tukey’s multiple comparison test. Results are shown to be means ± SEM