Fig. 8

IGLR facilitates diabetic wound closure through the COX2/PEG2 signaling pathway. A IF and FISH double staining for COX2 (green) and PGE2 (red) in wound tissue at Day 14 post surgery (scale bar, 50 μm). B, C Quantification of COX2 and PGE2 through IF and FISH analyses (n = 3). D, E PGE2 and IL-6 levels in plasma of diabetic mice at day 7 and 14 of gavage of different doses of IGLR; F–K COX2, IL-1β, IL-6, NLRP3, ASC and caspase1 mRNA expression within wound tissues detected by qRT-PCR after 7 and 14 days (n = 5). ^####P < 0.001 versus positive control group, *P < 0.05, **P < 0.01, ***P < 0.005 versus vehicle group, one-way ANOVA and Tukey’s multiple comparison test. Results are shown to be means ± SEM