Table 1 Primers used for marker analysis
| Technique | Annealing temperature (°C) | Name of the primer | Sequence | Number of polymorphic bands |
Marker length (kb) |
|---|---|---|---|---|---|
| RAPD | 35-40 | S62 | GTGAGGCGTC | 1 | 0.6 |
| S343 | TCGTGCGGGT | 2 | 0.9,1.1 | ||
| SCAR | 55-60 | SR-S343-F1 | CTCTTGAGCAATCCAAATGTTTTGTTATCA | ||
| SR-S343-R1 | CATAAATCACTTTATAACATAACGAGCTCGTATT | 1 | 1.03 | ||
| SR-S343-R2 | CGCGACAGAGGGGTTTTCTTTCTATTA | 1 | 0.95 | ||
| SR-S343-R3 | AGACGCCTCACTTTGATAGACATGAGTTTA | 1 | 0.89 | ||
| SNP | 50-60 | Sn-S62-a | CGACAGTAAACATAAAAACCG | 1 | 1* |
| Sn-S62-b | CTGTTACCATTGGCTCTTTACC | ||||
| CAPS | 60-67 | CP-S62-f | TCGTGCGGGTCATAGTACCCCGAGACAAGAGGCCAAAA | 2 | 0.25, 0.3 (Afl III) |
| CP-S62-r | TGTAATACGAAAGTTTAAGTCTCTTTTCTTAGTC | 0.2, 0.35 (Drd I) |