Development and validation of molecular markers for characterization of Boehmeria nivea var. nivea and Boehmeria nivea var. tenacissima

Table 1 Primers used for marker analysis

Technique	Annealing temperature (°C)	Name of the primer	Sequence	Number of polymorphic bands	Marker length (kb)
RAPD	35-40	S62	GTGAGGCGTC	1	0.6
RAPD		S343	TCGTGCGGGT	2	0.9,1.1
SCAR	55-60	SR-S343-F1	CTCTTGAGCAATCCAAATGTTTTGTTATCA
		SR-S343-R1	CATAAATCACTTTATAACATAACGAGCTCGTATT	1	1.03
		SR-S343-R2	CGCGACAGAGGGGTTTTCTTTCTATTA	1	0.95
		SR-S343-R3	AGACGCCTCACTTTGATAGACATGAGTTTA	1	0.89
SNP	50-60	Sn-S62-a	CGACAGTAAACATAAAAACCG	1	1*
SNP		Sn-S62-b	CTGTTACCATTGGCTCTTTACC
CAPS	60-67	CP-S62-f	TCGTGCGGGTCATAGTACCCCGAGACAAGAGGCCAAAA	2	0.25, 0.3 (Afl III)
CAPS		CP-S62-r	TGTAATACGAAAGTTTAAGTCTCTTTTCTTAGTC		0.2, 0.35 (Drd I)

* Only one base with different colors. The fluorescent dyes are assigned to the individual ddNTPs. Each ddNTP is labeled with a different color, ddATP green, ddCTP blue, ddGTP black, and ddTTP red.

ISSN: 1749-8546