Fig. 3

NF-κB activation is normal upon treatment with GTE. a Jurkat T cells were activated by PHA or PHA with GTE. Protein extracts were prepared at the indicated time points and subjected to western blot analysis. p-IκB and p-p65 protein levels were detected, and α-tubulin was used as an internal control. The densities of protein bands were determined by Image Lab software. Densitometric quantification of each protein band was normalized by the internal control. The levels of p-IκB and p-p65 are normalized by α-tubulin. Data represented fold activation from the non-activated (0 h) density (b, c)