Fig. 9

Schematic overview of Fe₃O₄@β₂-AR FPs-based ligand screening method. The Fe₃O₄@β₂-AR FPs were synthesized by immobilizing the receptor onto Fe₃O₄@NH₂ using N,N′-carbonyldiimidazole as a linking reagent. The bioactive compounds bound to β₂-AR were achieved by incubating the particles with desired samples and were isolated by a magnet. The bound compounds were released into solution by competitive displacement after the particles were totally rinsed. The compounds released from the particles were analyzed by LC–MS/MS to pursue their structures