Rapid screening and identification of bioactive compounds specifically binding to beta 2-adrenoceptor from San-ao decoction using affinity magnetic fine particles coupled with high-performance liquid chromatography–mass spectrometry

Table 1 Stability investigation of the immobilized β₂-AR MFPs

Times (day)	Salbutamol binding contents (ng)	Relative standard deviation (RSD)
1	58	2.1%
3	56
7	57
10	54
14	53

The binding contents of salbutamol were determined by denaturing the receptor using 10 mM glycine–HCl (pH 2.0) and releasing the drug into solution

ISSN: 1749-8546