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Inflammatory bowel disease: an overview of Chinese herbal medicine formula-based treatment

Chinese Medicine volume 17, Article number: 74 (2022) Cite this article

Abstract

Inflammatory bowel disease (IBD) is a chronic recurrent inflammatory disease of the intestine, including Crohn’s disease (CD) and ulcerative colitis (UC), whose etiology and pathogenesis have not been fully understood. Due to its prolonged course and chronic recurrence, IBD imposes a heavy economic burden and psychological stress on patients. Traditional Chinese Herbal Medicine has unique advantages in IBD treatment because of its symptomatic treatment. However, the advantages of the Chinese Herbal Medicine Formula (CHMF) have rarely been discussed. In recent years, many scholars have conducted fundamental studies on CHMF to delay IBD from different perspectives and found that CHMF may help maintain intestinal integrity, reduce inflammation, and decrease oxidative stress, thus playing a positive role in the treatment of IBD. Therefore, this review focuses on the mechanisms associated with CHMF in IBD treatment. CHMF has apparent advantages. In addition to the exact composition and controlled quality of modern drugs, it also has multi-component and multi-target synergistic effects. CHMF has good prospects in the treatment of IBD, but its multi-agent composition and wide range of targets exacerbate the difficulty of studying its treatment of IBD. Future research on CHMF-related mechanisms is needed to achieve better efficacy.

Background

Inflammatory bowel disease (IBD) is non-specific colitis, mainly including ulcerative colitis (UC) and Crohn’s disease (CD), with symptoms such as abdominal pain, diarrhea, and weight loss [1]. Currently, the prevalence of IBD is on the rise worldwide, with a prevalence of 10–20% in developed countries and more severe in developing countries [2, 3]. Pharmacological treatment of IBD in modern society mainly uses drugs such as 5-aminosalicylates and immunosuppressive agents. However, these drugs have side effects such as drug resistance and intestinal flora dysbiosis, which seriously affect the prognosis of patients’ lives [4]. Therefore, it is vital to investigate the pathological mechanism of IBD and find new targets and drugs for IBD treatment. The interaction between genetic and environmental factors, immune dysfunction, intestinal flora dysbiosis, and impaired intestinal mucosal barrier function is currently the leading causes of IBD development.

With the continuous development of the global pharmaceutical industry, Traditional Chinese Medicine has received more and more attention. Most Chinese Herbal Medicine has a long history of clinical experience in the treatment guided by Chinese medical theory. Chinese Herbal Medicine's development and exploitation procedure are the opposite of chemical drug research, which mainly starts with clinical practice screening to identify candidate compounds and then clinical validation to enter the new drug development track for clinical research, production, and marketing [5]. The Chinese Herbal Medicine Formula (CHMF) has certain specificities and advantages compared with some Western medicines. CHMF is a formula composed of two or more medicinal flavors, with relatively prescriptive processing and usage methods, for relatively definite disease evidence and is the main component of Traditional Chinese Medicine prescriptions. CHMF has a complex chemical composition and pharmacological effects with multi-target medical characteristics [6].

It has been shown that the co-administration of Indirubin and Isatin, the co-administration of Astragalus membranaceus polysaccharides, and Codonopsis pilosula polysaccharides, as well as the administration of Fuzi-Ganjiang, can improve clinical symptoms in UC mice [7,8,9]. The remarkable therapeutic advantages of Chinese medicine for IBD have received increasing attention from scholars, and this article describes the progress of basic research achieved by CHMF in the treatment of IBD in recent years. The current use of CHMF for IBD treatment is mainly focused on improving intestinal mucosal barrier function, immunomodulation, and regulating oxidative stress.

Intestinal mucosal barrier

Patients with IBD have a damaged intestinal mucosal barrier, leading to abnormal cytokine secretion, intestinal mucosal atrophy, permeability changes, and intestinal flora displacement [4]. This may lead to recurrent inflammation and aggravation of inflammation. The intestinal mucosal barrier comprises four parts: mechanical barrier, chemical barrier, immune barrier, and biological barrier. If one of the barriers is damaged, it will lead to the destruction of the intestinal mucosal barrier, resulting in impairment of its function, induction of intestinal infection, and the occurrence of diseases such as IBD.

Intestinal mucosal mechanical barrier

The mechanical barrier is the intestinal mucosal epithelial structure, including the intestinal epithelial cells (IECs) and the connections between IECs, which absorb nutrients and exclude harmful substances outside the barrier. The intestinal mucosal epithelial cells and the tight junction (TJ) between the cells form the intestinal mucosal mechanical barrier. The integrity of the intestinal mucosal mechanical barrier is determined by the composition and function of IECs and TJs [10]. The disruption of TJ infiltrates harmful luminal molecules and disrupts the intestinal mucosal immune system and inflammation. Therefore, TJ can act as a trigger for the development of intestinal and systemic diseases [11]. The TJ comprises a family of cytoplasmic proteins, like ZO (zonula occludens proteins), transmembrane proteins (tricellulin, nectin, junctional adhesion molecules, occludin, and claudins), and cytoskeletal structures together. Occludin and claudins can regulate the function of the intestinal mucosal mechanical barrier by affecting TJ permeability [12]. Targeting the restoration of TJs integrity may become an effective way to treat and prevent IBD.

In dextran sulfate sodium (DSS)-induced UC mice, the QingBai decoction, Huang-Lian-Jie-Du Decoction, and Huangqin Decoction increased the expression of ZO-1 and Occludin in colonic mucosal [13,14,15]. Fang investigated the effect of Qing Hua Chang Yin on the loss of intestinal epithelial barrier integrity induced by lipopolysaccharide (LPS) in vitro using a Caco-2 cell model and found that Qing Hua Chang Yin could upregulate the mRNA and protein expression levels of Claudin-1 [16]. In addition, Sijunzi Decoction can also upregulate the level of Claudin-2 in the colon tissue of rats induced by a 2,4,6-trinitrobenzene sulfonic acid (TNBS) in vivo as an intestinal barrier protector [17].

Intestinal mucosal chemical barrier

The mucosal chemical barrier is less reported, but it also plays an essential role in IBD. The loose mucus layer covering the intestinal epithelial cells and the mucin in the mucus together constitute the intestinal mucosal chemical barrier [18]. Mucins can prevent pathogenic bacteria from invading and adhering to the intestine. Huang-Lian-Jie-Du Decoction can protect the intestinal mucosa by increasing the secretion of mucins [14].

Intestinal mucosal microbial barrier

In recent years, intestinal flora disorders have become an essential factor in the pathogenesis of IBD. The micro spatial structure of the intestinal commensal bacteria and the host constitutes the intestinal mucosal microbial barrier. Intestinal flora can interact with cells to promote barrier function through nutrient acquisition, energy and metabolic regulation, and cell proliferation [19]. The relationship between the intestinal flora and the organism's autoimmune system coexists and influences each other. The imbalance of intestinal microecology can lead to various inflammatory and metabolic diseases in the body [20]. It has been shown that during the active phase of IBD, there is intestinal flora dysbiosis and decreased species diversity within the organism [21]. CHMF can regulate intestinal flora disorders and maintain the homeostatic balance of intestinal flora [22]. Huang-Lian-Jie-du Decoction, Huangqin Decoction, and Pyungwi-san can restore the balance of intestinal flora in UC mice by inhibiting the growth of intestinal pathogens and preventing the reduction of beneficial bacteria [23,24,25]. In DSS-induced UC rats, the ratio of Bacteroidetes to Firmicutes was elevated. This ratio decreased to normal levels with the use of Huai Hua San [26]. It has also been suggested that IBD is associated with Clostridium difficile (CDD) infection, resulting from dysbiosis of the intestinal flora. In contrast, Pyungwi-san normalizes the abundance ratio of Firmicutes/Bacteroidetes in the intestine and has some protective effect against DSS + CDD-induced colitis, which may be achieved by restoring the balance of the intestinal microbial community [27].

IBD can cause abnormal metabolic regulation in vivo. The resident intestinal flora can suppress the expression of pro-inflammatory cytokine genes by secreting short-chain fatty acids (SCFAs), vitamins, and other beneficial active metabolites [28]. Several metabolic pathways, including amino acids, fatty acids, and bile acids, are perturbed in IBD patients. Metabolomics characterizes the overall and dynamic changes in the type and concentration of endogenous small-molecule metabolites (e.g., amino acids, lipids, nucleosides) in the organism when disturbed by disease or drugs [29]. Subsequently, metabolomics can be correlated with genomic and proteomic results to facilitate the systematic study and comprehensive interpretation of disease mechanisms or drug mechanisms of action [30, 31]. Therefore, metabolomics is used to characterize the endogenous metabolic profiles of IBD patients and to reveal the critical metabolic pathways that are perturbed during the development and progression of IBD. It has important implications for the in-depth investigation of the pathogenesis of IBD and the search for new therapeutic targets for IBD. Hong analyzed the metabolic profile of UC rats by LC–MS/MS and identified a total of 36 differential metabolites involved in multiple metabolic pathways. Compound Sophorae Decoction could affect multiple metabolic pathways in TNBS-induced UC rats, thus diminishing their pathophysiological symptoms and obtaining positive therapeutic effects [32]. Huankuile Suspension inhibits inflammatory response and regulates bile metabolism, pyrimidine metabolism, purine metabolism, glutathione metabolism, and citric acid cycle in UC rats [33]. Huang-Lian-Jie-du decoction alleviates UC in mice by regulating arachidonic acid metabolism and glycerophospholipid metabolism [34]. Astragalus membranaceus polysaccharides and Codonopsis pilosula polysaccharides could upregulate the effects of isovaleric and butyric acids in SCFAs to improve clinical symptoms in mice with colitis [9]. Huangqin Decoction plays a crucial role in normalizing metabolic disorders by regulating the levels of amino acid, lipid, and fatty acid markers in UC rats [35, 36]. Rhubarb Peony Decoction increased the number of butyric acid-producing Butyricicoccus pullicaecorum and the level of SCFAs to restore intestinal function in UC mice. The dynamic changes of intestinal flora and host co-metabolites were detected by metabolomics to clearly demonstrate the metabolic status of intestinal flora in the host, which may provide clues and directions for studies such as the drug treatment mechanism IBD [37].

Intestinal mucosal immune barrier

Gut-associated lymphoid tissue (GALT), secretory antibodies, and mesenteric lymph nodes (ETC) constitute the intestinal mucosal immune barrier, which can respond to antitoxins, antigens, and potentially harmful organisms [38]. GALT produces IgA, which forms the antigen complexes with antigenic material, binds to receptors on M cells, and antigens are transferred to the lamina propria and then presented to dendritic cells (DCs). Inflammatory DCs play an essential role in the pathogenesis of IBD, and Sishen Pill can modulate the interaction between inflammatory DCs and the gut microbiota to treat DSS-induced colitis [39].

Immunity

T lymphocytes

The exact mechanism of cellular dysfunction leading to IBD is not fully understood. However, modulating the function of immune cells could be a powerful tool in the treatment of inflammatory diseases. The adaptation of T lymphocytes to the intestinal environment requires constant differentiation between natural stimuli from commensal flora, natural stimuli from food, and pathogens that need to be removed. T lymphocytes in the gut can be activated by environmental and other factors, causing genetic variants of intestinal defense defects or tolerance disruptions, such as intestinal infections or flora imbalances, triggering multiple immune disorders [40]. Therefore, T lymphocytes are the critical site of drug action for IBD treatment. The intestinal inflammatory infiltrate is mainly composed of CD4+ T cells, regulatory T cells (Tregs), and main memory T Cells (Tcm) [41]. There are several specific subpopulations of T helper cells: Th1, Th2, Th9, Th17, Th22, T follicular helper (Tfh), and several auto Tregs [41]. Th17 cells play an essential barrier role in the skin and intestinal mucosa. The resistance to bacteria and fungi is an essential driver of autoimmune disease, which often exacerbates disease when triggered in the autoimmune environment. The differentiation of Th17 is mainly driven by interleukin (IL)-6 and transforming growth factor (TGF)-β, which is further stabilized by paracrine signals such as IL-23 and IL-1β [42]. It is now generally accepted that the solid anti-inflammatory CD4+ T-cell, Tregs, are abundant in the intestine [43]. In DSS-induced UC mice, Huangqin Decoction was able to increase the number of Tregs to alleviate the inflammatory effects [15]. The role of Tregs cells is mainly to suppress inflammation by suppressing T cells and regulating other immune cells in their environment, especially interconnected with Th17 cells in differentiation, which together maintain the body's balance of the immune microenvironment.

Once this balance is disrupted, multiple autoimmune diseases, including IBD, can occur [44]. In exploring the relationship between T lymphocytes and IBD, it has been found that Gegen Qinlian Decoction could restore the balance of Treg and Th17 cells in the colonic tissue of UC mice [45]. In TNBS-induced mouse mesenteric lymph node lymphocytes and lamina propria monocytes, Qingre Zaoshi Liangxue Decoction decreased the proportion of Th17 cells and increased the proportion of Treg cells [46]. Compound Sophorae Decoction can regulate the percentage of Th17 and Treg cells in the mesenteric lymph nodes of UC mice [47]. Furthermore, Bawei Xileisan restored the balance of Th17/Treg in monocytes [48]. The role of Tfh cells is to participate in B-cell differentiation and play a role in immunoglobulin production and the formation of lymphocyte tissue-growing centers. Sishen Pill effectively treated chronic colitis by regulating Tfh cell differentiation and function to treat IBD [49].

NF-κB

The nuclear factor kappa B (NF-κB) family includes NF-κB1 (p50 and precursor p105), NF-κB2 (p52 and precursor p100), and related factor A, nuclear factor c-Rel, and related factor B. They all have a Rel homologous structural domain at the N terminus, which dimerizes with the DNA sequence that specifically binds to DNA sequences and activates or represses downstream transcription [50]. In the absence of external signal stimulation, the above proteins form homodimers or heterodimers and are strictly inhibited by the inhibitor of the NF-κB (IκB) family. They are regulated by the inhibitor of the NF-κB kinase (IKK) complex [51]. NF-κB is a crucial regulator of inflammation and can be activated by various stimulatory factors. The degradation of IκB translocates NF-κB to the nucleus and mediates the transcription of various target genes. Several pro-inflammatory factors encoded by the NF-κB signaling pathway promote inflammation-associated tissue damage and are associated with tumor development [52]. Huangkui Lianchang Decoction inhibits the NF-κB signaling pathway in DSS-induced ulcerative colitis [53]. Jian-Pi Qing-Chang Decoction and Tou Nong San can improve mucosal inflammatory response and intestinal epithelial barrier function through the NF-κB pathway. Also, their anti-inflammatory effects are associated with NF-κB regulation [54,55,56,57].

TOLL-like receptors (TLRs) are cellular transmembrane receptors in the natural immune system. TLRs can bind to pathogen recognition pattern molecules, activate downstream signaling molecules, and ultimately trigger the expression of inflammatory mediators, serving as a link between natural immunity and acquired immunity. TLR4 is a subtype of the TOLL receptor family, and myeloid differentiation factor 88 (MyD88) is the main junction protein in the TLR4 signaling pathway. The interaction between TLR4 and MyD88 ultimately mediates the innate immune response and inflammatory response against pathogenic bacteria [58]. It was found that the TLR4 mediated signaling pathway is involved in the development of UC. TLR4 is a transmembrane receptor in the natural immune system, which recognizes the corresponding ligand and then binds to it to initiate signal transduction, leading to NF-κB activation and release of intestinal inflammatory mediators [59]. Gegen Qinlian Decoction can inhibit the TLR4/NF-κB signaling [60]. Kuijieyuan Decoction ameliorates intestinal barrier damage in ulcerative colitis by affecting TLR4-dependent NF-κB signaling [61].

Myosin light chain kinase (MLCK) is a calmodulin-dependent serine/threonine-specific protein kinase whose primary function is to phosphorylate myosin light chain (MLC) and activate myosin heavy chain adenosine triphosphatase. MLCK mediates the sliding of skeletal protein microfilaments, causing cell contraction and eventually the formation of cellular gaps [62]. A variety of signaling pathways are involved in the activation of MLCK, mainly through the mitogen-activated protein kinase (MAPK) pathway of extracellular regulated protein kinase (ERK) 1/2, P38, c-Jun N-terminal kinase (JNK) pathway, as well as inflammation-related pathways involved in cell proliferation and apoptosis [63]. In TNBS-induced UC rats, Bu-Zhong-Yi-Qi Granule can regulate the secretion of some inflammatory cytokines and improve TJ integrity through TLR4/NF-κB/MLCK pathway [64]. Sijunzi Decoction could reduce the levels of NF-κB p65 and MLCK [17]. In addition, Shen-Ling-Bai-Zhu-San attenuated DSS-induced UC mice via the MAPK/NF-κB signaling pathway [65]. NEMO is encoded by the B cell-encoded κ light chain polypeptide repressor gene (IκBKG) [66], and in 1998, Brott cloned the mammalian homolog of the Nemo gene named Nemo-like kinase (NLK) [67]. It has been confirmed that NLK is a highly conserved MAPK-like kinase during evolution and has a significant reference value for disease prognosis [68]. Sishen Pill can inhibit NF-κB activation by suppressing the NEMO/NLK signaling pathway and plays a vital role in treating chronic colitis [69].

The assembly of NOD-like receptor protein 3 (NLRP3) requires the involvement of the sensor NLRP3 pattern recognition receptor, apoptosis-associated speck-like protein containing a CARD (ASC), and the effector protein caspase-1 are involved, which widely presented in various immune cells when the disease occurs [70]. Microbial molecules require the activation of NLRP3 or some signaling through NF-κB proteins, which induce NLRP3 to indirectly promote the expression of the assembled inflammasome complex [71]. Both Jiaweishaoyao Decoction and Pyungwi-san can inhibit the NLRP3 inflammasome, and the NF-κB pathway to alleviate DSS-induced UC [72, 73].

PI3K is an intracellular phosphatidylinositol kinase. Upon the activation of PI3K, PIP3 acts as a second messenger and binds to regional proteins of Akt. This leads to the acquisition of Akt activity, which is involved in cell growth, cell development, and apoptosis through the regulation of downstream proteins [74]. Akt is a serine/threonine kinase, also known as protein kinase B or PKB, which is a target of action downstream of the PI3K pathway, and its anti-apoptosis mechanism is phosphorylation of target proteins through multiple downstream pathways. The activated Akt is involved in activating and inhibiting multiple targets after phosphorylation, enabling cell survival, growth, and proliferation through multiple mechanisms [75]. Zuojin Pill can modulate the crosstalk between intestinal microbes and Treg cells to attenuate DSS-induced colitis through PI3K/Akt signaling pathway [76]. Sishen Pill alleviates DSS-induced colitis, which may be related to inhibiting the PI3K/Akt signaling pathway [77]. Xianglian Pill can block the activation of the PI3K/Akt/mTOR pathway, inhibit the secretion of pro-inflammatory cytokines, and repair the dysfunction of the intestinal epithelial barrier to enhance autophagy [78]. Upon activating the PI3K/Akt pathway, activated Akt inhibits phosphorylation degradation of protein IκB kinase by enhancing NF-κB, which subsequently leads to NF-κB activation. Huangqin Decoction can improve DSS-induced colitis by modulating the intestinal microbiota and inhibiting the PI3K/Akt/HIF-1α and NF-κB pathways [79]. Kuijieyuan Decoction ameliorates intestinal barrier damage in ulcerative colitis by affecting TLR4-dependent PI3K/AKT/NF-κB signaling pathway [61].

JAK2/STAT3

The JAK/STAT3 signaling pathway is composed of Janus kinase (JAK), tyrosine kinase receptor, and Signal Transducer and Activator of Transcription 3 (STAT3), which are essential in cell growth, cell proliferation, cell invasion, cell metastasis, and regulation of apoptotic processes [80]. The STAT family, a group of intracellular proteins that signal and activate transcriptional functions, contains seven members (STAT1-4, 5A, 5B, and 6). The JAK2/STAT3 signaling pathway is an important pathway mediating the signaling of numerous cytokines and inflammatory mediators, closely associated with the expression of inflammatory immune factors associated with tumor necrosis factor-α (TNF-α), IL-6, IL-17, and IL-22 [81]. The activation of JAK2 will phosphorylate tyrosine residues, which are later bound to the receptor and phosphorylated by STAT3. Phosphorylated JAK2 forms a dimer or heterodimer with phosphorylated STAT3 and ectopic to the nucleus, affecting the transcription of downstream genes and the expression of inflammatory factors [82]. Gegen Qinlian Decoction and Huanglian Jiedu Decoction reduce inflammation by inhibiting JAK2/STAT3, decrease inflammation by inhibiting JAK2/STAT3 signaling, and has a protective effect on UC [45, 83]. Qingre Zaoshi Liangxue Decoction, Pien Tze Huang, and Baitouweng Decoction significantly improved the inflammatory symptoms in mice with acute colitis, and the latent mechanism may be related to various signaling pathways, including regulation of gut microbiota and inflammatory signaling pathways, such as IL-6/STAT3 [46, 84, 85].

Notch

The Notch pathway is highly conserved in various organisms, is involved in developing almost all organ systems, and regulates tissue homeostasis after development. The Notch signaling pathways include Notch ligands (Delta-like ligands 1, 3, 4, Serrate-like ligands Jagged1, Jagged2), Notch1, Notch2, Notch3, Notch4, Notch DNA binding proteins, immunoglobulin κJ region recombinant signaling proteins and effector molecules (Hes, Hcy, Herp) [86]. The notch signaling pathway activates Notch receptors on the cell surface upon binding to ligands, inducing protein hydrolase cleavage. The intracellular segment of Notch is released into the nucleus and binds to the transcriptional repressor RBP-Jκ to activate the transcription of target genes, which can regulate cell proliferation, cell differentiation, and apoptosis [87]. In the DSS-induced UC mice, Gegen Qinlian Decoction maintains mucosal homeostasis through bidirectional regulation of Notch signaling, thereby restoring colonic epithelial function [88]. Compound Sophorae Decoction modulates the Notch signaling, promotes altered macrophage phenotype, and enhances colonic mucosal barrier function [89]. QingBai Decoction inhibited the effects of NF-κB and Notch signaling on the inflammatory cascade, which effectively alleviated intestinal inflammation and mucosal barrier function in DSS-induced UC mice [13].

Others

TLRs are vital pattern recognition receptors that can lead to uncontrolled inflammatory responses in case of excessive activation [90]. IL-1 receptor-associated kinase (IRAK) mediates multicellular receptor signaling, including TLRs, and has an essential regulatory role in various inflammatory cell signaling networks [91]. Tiaochang Xiaoyan Extract Tablets improved colonic inflammation in rats with chronic colitis, and this effect may be achieved by activating lysosomes in macrophages through inhibition of the TLR9/MyD88/IRAK signaling pathway [92].

The primary biological function of Oncostatin-M (OSM) is to inhibit the growth of a variety of tumor cells and to induce the differentiation of specific tumor cells. OSM is a promising cytokine because it can significantly inhibit the growth and induce the differentiation of tumor cells through a variety of different pathways.

The Oncostatin-M receptor (OSMR) is widely distributed on the surface of many tumor cells, endothelial cells, and epithelial cells [93]. The expression of OSM and OSMR is increased in intestinal tissues of patients with IBD, which leads to increased intestinal inflammation. Therefore, OSM and OSMR have become markers in IBD diagnosis [94]. Feiyangchangweiyan Capsule affects UC by inhibiting the OSM/OSMR pathway and regulating inflammatory factors to improve intestinal flora [95].

The Wnt signaling pathway regulates the growth and development of the body, which maintains the normal physiological functions of many tissues and organs, and its abnormal activation or inhibition contributes to the development of many diseases. The Wnt/β-catenin signaling pathway is one of the most important signaling pathways that significantly impact the progression of multiple diseases. Its central role is to activate the proliferation of intestinal stem cells and inhibit the differentiation of intestinal stem cells from maturing cell types [96, 97]. Sishen Pill effectively attenuated TNBS-induced colitis, which inhibited the Wnt/β-catenin signaling pathway [77].

Oxidative stress

It has been reported that the concentration of reactive oxygen species (ROS) in intestinal cells increases during chronic inflammatory and recurrent immune responses in the gut as the disease develops [98]. ROS regulates cellular regulation by oxidizing DNA, proteins, lipids, and other cellular structures. In order to protect biological systems from damage caused by excessive ROS, the cellular antioxidant system is activated and thus regulates ROS production. ROS levels were significantly increased in the colon of DSS-induced UC mice. Gegen Qinlian decoction and Sanhuang Shu'ai decoction reduced ROS concentrations and thus prevented the onset and progression of the disease [60, 99]. Nuclear factor E2-related factor 2 (Nrf2) is an important transcription factor regulating the cellular oxidative stress response and is a central regulator in maintaining intracellular redox homeostasis. Nrf2 regulates constitutive and inducible expression of a series of antioxidant proteins to mitigate reactive oxygen species and electrophilic body-induced cellular damage and maintain tissue cell redox dynamic homeostasis [100]. Huang-Lian-Jie-Du Decoction can effectively alleviate DSS-induced UC mice by inhibiting the NF-κB signaling pathway, activating the Nrf2 signaling pathway, and enhancing intestinal barrier function [14]. Superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) are downstream enzymes of the Nrf2 pathway that convert oxygen radicals into hydrogen peroxide and rapidly break them down into the water, effectively preventing tissue cells from being damaged by peroxides [101]. SOD can scavenge superoxide anions produced in the body. CAT is a crucial component of the enzyme system in the body’s antioxidant system, which can scavenge free radicals in synergy with SOD, and GSH can protect cell membrane structure and function from oxidative damage by hydrogen peroxide. All SOD, CAT, and GSH have essential roles as antioxidants, and when their levels and activity are reduced, they lead to the accumulation of free radicals [102]. Myeloperoxidase (MPO) is a crucial peroxidase produced by neutrophil asplenophil granules, and its activity level reflects the degree of neutrophil infiltration. Malondialdehyde (MDA) is the end product of the lipid peroxidation reaction between ROS and unsaturated fatty acids on the cell membrane, and its level can indirectly reflect the degree of lipid peroxidation [102]. Gegen Qinlian Decoction and Kuijieyuan Decoction reduced MDA and MPO levels and increased SOD, GSH, and CAT levels, showing a protective function against UC animal models [60, 61].

Novel Chinese herbal medicine formula

As a unique health resource in China, Chinese Medicine is increasing attention worldwide. In novel drug development, Chinese Herbal Medicine and natural products have become an essential source of innovative drug development. At present, for the research and evaluation of new drugs in different categories of Chinese Medicine and natural drugs, based on many years of experience in new drug research in Chinese medicine, many people have proposed ideas for the research and evaluation of drugs in different categories originating from classical formulas, and combined with basic research to carry out innovative research in various aspects.

Qingre Jianpi Decoction, the classically formulated optimized Qingchang Suppository, and the simplified prescription Suqing Pill reduced the secretion of inflammatory cytokines and exerted anti-inflammatory effects in UC treatment [103,104,105]. The Chinese herbal standardized product Guchang Capsule, Semi-bionic Extraction of compound Turmeric, Modified Pulsatilla Decoction, the functional beverage Ampelopsis grossedentata, and Guchang Zhixie Pill can all exert protective effects against IBD via the NF-κB signaling pathway [106,107,108,109,110].

Chemokine receptor 3 (CXCR3) is a specific binding protein that inhibits the endogenous chemokine IP10, and the IP10/CXCR3 axis plays a vital role in the pathogenesis of childhood IBD, and inhibition of IP10 may alleviate the clinical symptoms of UC [111]. CRCX3 is activated once it binds to IP10 and contributes to its transfer to the inflammatory localization for further effects. The large amount of inflammatory cytokines produced by local inflammation of intestinal mucosa stimulates IP10 to recruit more inflammatory cells to form a cascade response and aggravates the damage of the intestinal mucosal barrier [112]. Qingchang Wenzhong Decoction was able to downregulate IP10/CXCR3 axis-mediated inflammatory response, improve DSS-induced UC in rats, and maybe a new UC therapy [113]. Aquaporins (AQPs) are essential proteins in the body's aqueous metabolism process, mediating the transport of water molecules along an osmotic gradient across cell membranes and mediating the transport of water molecules across different cell membranes [114]. AQPs are mainly involved in regulating urine concentration and fluid permeation in humans [115]. Ershen Pill Extract effectively improved diarrhea in rats by improving the synthesis of AQP3 in the colon [116]. In DSS-induced colitis, the increase in vascular permeability precedes the increase in intestinal epithelial permeability. Qingchang Suppository can reduce colonic vascular permeability and improve vascular endothelial barrier function by modulating the VEGF/HIF-1α signaling pathway [117]. In addition, some novel herbal combinations play an essential role in IBD. The use of Qingchang Suppository reduced colonic tissue edema, vascular congestion, and inflammatory cell infiltration [117]. Costus root granules significantly improved inflammation and apoptosis in the colonic epithelium by modulating the transforming growth factor (TGF)-β-mediated PI3K/AKT signaling pathway [118]. Xinhuang Tablets alleviated DSS-induced UC in mice by increasing intestinal epithelial TJ expression [119]. Composite Sophora Colon-Soluble Capsule also showed significant effects on UC from restoring intestinal microbiota and intestinal immune homeostasis [120].

The CHMF prepared by different methods can provide vital information for further mechanistic exploration of traditional prescriptions, contributing to the rational application of herbal compounding in modern applications or scientific research and improving human knowledge of herbal compounding. Banxia Xiexin Decoction is widely used in modern clinical practice. Both modern and ancient extraction methods can alleviate the severity of UC rats to different degrees [121]. The differences in the efficacy of the five Ganjiang Decoction extracts on DSS-induced UC in mice were closely related to the extraction methods. The study by Wei improved the extraction process of Ganjiang Decoction, which provided the basis for the process of enteric preparation and offered new ideas for the compounding of Chinese Herbal Medicines [122]. These Novel Chinese Herbal Medicine Formulas provide reference and reference for the development of new drugs in Chinese Medicine and natural products, intending to improve the efficiency and success rate of new drug development and enhance the development of new products.

Conclusions

The development of new drugs for CHMF is a crucial area of modern research in Chinese medicine. Chinese medicines, especially CHMF, are different from chemical, biological, and botanical drugs. Due to the complexity of the pharmacological substances and targets or links contained in CHMF, it poses a great difficulty for new drug development, but at the same time, it also has significant room for innovation. At present, the clinical treatment of IBD is mainly based on immunosuppressive drugs or hormone therapy, and the use of these drugs is strictly regulated, which is prone to adverse reactions and drug resistance if used irregularly. Table 1 shows the targets of the Chinese Herbal Medicine Formula in attenuating IBD. We hope to explore the association between CHMF and IBD to find new ideas for new drug development. At present, we should be oriented to highlight the characteristics and advantages of CHMF efficacy, fully utilize and draw on modern biotechnology, and thoroughly study the scientific and technological issues related to CHMF development. The level of knowledge of CHMF pharmacological substances and mechanisms of action should be further understood to contribute to creating high-level new drugs and provide new directions and ideas for the treatment of IBD.

Table 1 The targets of the Chinese Herbal Medicine Formula in attenuating IBD
Full size table

In the West, biological agents are the fastest-growing segment of the prescription drug market and cost thousands of dollars per patient per year to treat IBD. There is a need to reconcile the most appropriate treatment for these patient populations from the perspectives of both disease presentation and cost. In developing countries, healthcare systems and indeed the patients struggle to afford such expensive treatments [123].

The classics of traditional Chinese medicine are summaries of the rich practical experience of physicians in the past dynasties, and they are the standard to guide the clinical practice of traditional Chinese medicine for thousands of years. By collecting and arranging medical records and herbal medicines of past dynasties, combined with folk prescriptions and prescriptions offered by famous doctors, traditional Chinese medicines and their compound prescriptions with apparent curative effect, wide clinical application, apparent characteristics, and advantages are sorted out. On this basis, the experimental research of screening traditional Chinese medicines with various pharmacological models is carried out, and modern multidisciplinary technology is used to reveal the pharmacodynamic material basis and molecular mechanism of traditional Chinese medicines and then promote the development of innovative traditional Chinese medicines and their application in clinical practice. For example, arsenic trioxide, developed from the traditional Chinese medicine arsenic for treating acute promyelocytic leukemia (APL), has a good effect [124]. The excavation and development of classic prescriptions is the entry point and breakthrough point for the inheritance and innovation of traditional Chinese medicine in the new era. The classic recipes derived from ancient books have thousands of years of human experience and are widely used in common diseases, frequently-occurring diseases, chronic diseases, and other fields. The research and development of classic prescriptions is a fast and effective transformation path, effectively filling the gaps in medical drugs for some chronic and intractable diseases. The commonly used CHMF originates from clinical practice and has experienced thousands of years of inheritance and practice. However, these CHMFs are challenging to be recognized by the public at home and abroad. For the widely used clinical prescriptions with exact curative effects, the world-recognized methods and scientific data are used to demonstrate their clinical efficacy and safety. Their clinical positioning is precise, which is conducive to the broader and more effective clinical use of traditional Chinese medicine.

The elucidation of the material basis of compound recipes is of great significance to the research on the mechanism of action of traditional Chinese medicine, quality control, new drug research, and new drug development. In recent years, modern scholars, based on the holistic view of traditional Chinese medicine, combined the spectrum-effect relationship of traditional Chinese medicine theory and bioinformatics to clarify the pharmacodynamic substances as well as their mechanism of action and achieved a transformation. Identify the pharmacodynamic substances, explain the interaction between CHMF and the body, and then use pharmacological methods to study the mechanism of action. Considering a single factor's physical and chemical factors, the traditional Chinese medicine etiological animal model induced by biological damage, the Western medicine etiological and pathological animal model, and the clinical application characteristics of Chinese medicine cannot be well integrated. The researchers further established a disease-syndrome combination model to study the material basis and mechanism of CMHF. The combination of disease and syndrome model can reflect the characteristics of traditional Chinese medicine and be supported by the pathological changes and diagnostic evaluation of diseases in Western medicine. It is a trend in modern Chinese medicine research models. In this way, the pharmacodynamic material basis and mechanism of CHMF can be analyzed from the clinical point of view, the nature of the disease, and the overall composite level.

Availability of data and materials

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Abbreviations

IBD:

Inflammatory bowel disease

CD:

Crohn’s disease

UC:

Ulcerative colitis

CHMF:

Chinese herbal medicine formula

IECs:

Intestinal epithelial cells

TJ:

Tight junction

ZO:

Zonula occludens proteins

DSS:

Dextran sulfate sodium

LPS:

Lipopolysaccharide

TNBS:

2,4,6-Trinitrobenzene sulfonic acid

CDD:

Clostridium difficile

SCFAs:

Short-chain fatty acids

GALT:

Gut-associated lymphoid tissue

DCs:

Dendritic cells

Tfh:

T follicular helper

IL:

Interleukin

TGF:

Transforming growth factor

NF-κB:

Nuclear factor kappa B

IκB:

Inhibitor of the NF-κB

IKK:

Inhibitor of NF-κB kinase

TLRs:

TOLL-like receptors

MyD88:

Myeloid differentiation factor 88

MLCK:

Myosin light chain kinase

MLC:

Myosin light chain

MAPK:

Mitogen-activated protein kinase

ERK:

Extracellular regulated protein kinase

JNK:

Jun N-terminal kinase

IκBKG:

κ Light chain polypeptide repressor gene

NLK:

Nemo-like kinase

nlrP3:

NOD-like receptor protein 3

ASC:

Apoptosis-associated Speck-like protein containing a CARD

JAK:

Janus kinase

STAT3:

Signal transducer and activator of transcription 3

TNF-α:

Tumor necrosis factor-α

IRAK:

IL-1 receptor-associated kinase

OSM:

Oncostatin-M

OSMR:

Oncostatin-M receptor

ROS:

Reactive oxygen species

Nrf2:

Nuclear factor E2-related factor 2

SOD:

Superoxide dismutase

CAT:

Catalase

GSH:

Glutathione

MPO:

Myeloperoxidase

MDA:

Malondialdehyde

AQPs:

Aquaporins

References

  1. Al-Mustanjid M, Mahmud SMH, Royel MRI, Rahman MH, Islam T, Rahman MR, et al. Detection of molecular signatures and pathways shared in inflammatory bowel disease and colorectal cancer: a bioinformatics and systems biology approach. Genomics. 2020;112(5):3416–26.

    Article  CAS  PubMed  Google Scholar 

  2. Ibrahim S, Zhu X, Luo X, Feng Y, Wang J. PIK3R3 regulates ZO-1 expression through the NF-kB pathway in inflammatory bowel disease. Int Immunopharmacol. 2020;85: 106610.

    Article  CAS  PubMed  Google Scholar 

  3. Linson EA, Hanauer SB. Epidemiology of colorectal cancer in inflammatory bowel disease—the evolving landscape. Curr Gastroenterol Rep. 2021;23(9):16.

    Article  PubMed  Google Scholar 

  4. Zeeshan M, Ali H, Khan S, Khan SA, Weigmann B. Advances in orally-delivered pH-sensitive nanocarrier systems; an optimistic approach for the treatment of inflammatory bowel disease. Int J Pharm. 2019;558:201–14.

    Article  CAS  PubMed  Google Scholar 

  5. Li H. Advances in anti hepatic fibrotic therapy with traditional Chinese medicine herbal formula. J Ethnopharmacol. 2020;251: 112442.

    Article  CAS  PubMed  Google Scholar 

  6. Luan X, Zhang LJ, Li XQ, Rahman K, Zhang H, Chen HZ, et al. Compound-based Chinese medicine formula: from discovery to compatibility mechanism. J Ethnopharmacol. 2020;254: 112687.

    Article  CAS  PubMed  Google Scholar 

  7. Gao W, Zhang L, Wang X, Yu L, Wang C, Gong Y. The combination of indirubin and isatin attenuates dextran sodium sulfate induced ulcerative colitis in mice. Biochem Cell Biol. 2018;96(5):636–45.

    Article  CAS  PubMed  Google Scholar 

  8. Huang C, Dong J, Jin X, Ma H, Zhang D, Wang F, et al. Intestinal anti-inflammatory effects of fuzi-ganjiang herb pair against DSS-induced ulcerative colitis in mice. J Ethnopharmacol. 2020;261: 112951.

    Article  CAS  PubMed  Google Scholar 

  9. Tang S, Liu W, Zhao Q, Li K, Zhu J, Yao W, et al. Combination of polysaccharides from Astragalus membranaceus and Codonopsis pilosula ameliorated mice colitis and underlying mechanisms. J Ethnopharmacol. 2021;264: 113280.

    Article  CAS  PubMed  Google Scholar 

  10. Epple HJ, Zeitz M. HIV infection and the intestinal mucosal barrier. Ann N Y Acad Sci. 2012;1258:19–24.

    Article  CAS  PubMed  Google Scholar 

  11. Lee SH. Intestinal permeability regulation by tight junction: implication on inflammatory bowel diseases. Intest Res. 2015;13(1):11–8.

    Article  PubMed  PubMed Central  Google Scholar 

  12. Takizawa Y, Kishimoto H, Tomita M, Hayashi M. Changes in the expression levels of tight junction components during reconstruction of tight junction from mucosal lesion by intestinal ischemia/reperfusion. Eur J Drug Metab Pharmacokinet. 2014;39(3):211–20.

    Article  CAS  PubMed  Google Scholar 

  13. Lin JC, Wu JQ, Wang F, Tang FY, Sun J, Xu B, et al. QingBai decoction regulates intestinal permeability of dextran sulphate sodium-induced colitis through the modulation of notch and NF-kappaB signalling. Cell Prolif. 2019;52(2): e12547.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  14. Yuan Z, Yang L, Zhang X, Ji P, Hua Y, Wei Y. Huang-Lian-Jie-Du decoction ameliorates acute ulcerative colitis in mice via regulating NF-kappaB and Nrf2 signaling pathways and enhancing intestinal barrier function. Front Pharmacol. 2019;10:1354.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Zou Y, Lin J, Li W, Wu Z, He Z, Huang G, et al. Huangqin-tang ameliorates dextran sodium sulphate-induced colitis by regulating intestinal epithelial cell homeostasis, inflammation and immune response. Sci Rep. 2016;6:39299.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Fang W, Zhao P, Shen A, Liu L, Chen H, Chen Y, et al. Effects of Qing Hua Chang Yin on lipopolysaccharide induced intestinal epithelial tight junction injury in Caco2 cells. Mol Med Rep. 2021;23(3):1.

    PubMed  Google Scholar 

  17. Lu Y, Lin H, Zhang J, Wei J, Sun J, Han L. Sijunzi decoction attenuates 2, 4, 6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in rats and ameliorates TNBS-induced claudin-2 damage via NF-kappaB pathway in Caco2 cells. BMC Complement Altern Med. 2017;17(1):35.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  18. Rodriguez-Pineiro AM, Bergstrom JH, Ermund A, Gustafsson JK, Schutte A, Johansson ME, et al. Studies of mucus in mouse stomach, small intestine, and colon. II. Gastrointestinal mucus proteome reveals Muc2 and Muc5ac accompanied by a set of core proteins. Am J Physiol Gastrointest Liver Physiol. 2013;305(5):G348–56.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Albert-Bayo M, Paracuellos I, Gonzalez-Castro AM, Rodriguez-Urrutia A, Rodriguez-Lagunas MJ, Alonso-Cotoner C, et al. Intestinal mucosal mast cells: key modulators of barrier function and homeostasis. Cells. 2019;8(2):135.

    Article  CAS  PubMed Central  Google Scholar 

  20. Muzes G, Tulassay Z, Sipos F. Interplay of autophagy and innate immunity in Crohn’s disease: a key immunobiologic feature. World J Gastroenterol. 2013;19(28):4447–54.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  21. Wang W, Chen L, Zhou R, Wang X, Song L, Huang S, et al. Increased proportions of Bifidobacterium and the Lactobacillus group and loss of butyrate-producing bacteria in inflammatory bowel disease. J Clin Microbiol. 2014;52(2):398–406.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  22. Jiang H, Qu L, Dou R, Lu L, Bian S, Zhu W. Potential role of mesenchymal stem cells in alleviating intestinal ischemia/reperfusion impairment. PLoS ONE. 2013;8(9): e74468.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Yang Y, Chen G, Yang Q, Ye J, Cai X, Tsering P, et al. Gut microbiota drives the attenuation of dextran sulphate sodium-induced colitis by Huangqin decoction. Oncotarget. 2017;8(30):48863–74.

    Article  PubMed  PubMed Central  Google Scholar 

  24. Yuan Z, Yang L, Zhang X, Ji P, Wei Y. Therapeutic effect of n-butanol fraction of Huang-lian-Jie-du decoction on ulcerative colitis and its regulation on intestinal flora in colitis mice. Biomed Pharmacother. 2020;121: 109638.

    Article  CAS  PubMed  Google Scholar 

  25. Zhang Z, Cao H, Shen P, Liu J, Cao Y, Zhang N. Ping weisan alleviates chronic colitis in mice by regulating intestinal microbiota composition. J Ethnopharmacol. 2020;255: 112715.

    Article  CAS  PubMed  Google Scholar 

  26. Liu P, Bian Y, Liu T, Zhong J, Zhong Y, Zhuang S, et al. Huai hua san alleviates dextran sulphate sodium-induced colitis and modulates colonic microbiota. J Ethnopharmacol. 2020;259: 112944.

    Article  CAS  PubMed  Google Scholar 

  27. Yang M, Bose S, Lim SK, Kim H. Preventive effects of Pyungwi-san against dextran sulfate sodium- and Clostridium difficile-induced inflammatory bowel disease in mice. Int J Mol Sci. 2019;20(24):6346.

    Article  CAS  PubMed Central  Google Scholar 

  28. Singh N, Gurav A, Sivaprakasam S, Brady E, Padia R, Shi H, et al. Activation of Gpr109a, receptor for niacin and the commensal metabolite butyrate, suppresses colonic inflammation and carcinogenesis. Immunity. 2014;40(1):128–39.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. de Preter V. Metabolomics in the clinical diagnosis of inflammatory bowel disease. Dig Dis. 2015;33(Suppl 1):2–10.

    Article  PubMed  Google Scholar 

  30. Bottcher T, Pitscheider M, Sieber SA. Natural products and their biological targets: proteomic and metabolomic labeling strategies. Angew Chem Int Ed Engl. 2010;49(15):2680–98.

    Article  PubMed  CAS  Google Scholar 

  31. Dehairs J, Derua R, Rueda-Rincon N, Swinnen JV. Lipidomics in drug development. Drug Discov Today Technol. 2015;13:33–8.

    Article  PubMed  Google Scholar 

  32. Hong ZC, Cai Q, Wu HZ, Yang YF, Fan H, Duan XY. Compound Sophorae decoction: treating ulcerative colitis by affecting multiple metabolic pathways. Chin J Nat Med. 2021;19(4):267–83.

    CAS  PubMed  Google Scholar 

  33. Han Z, Wang H, Guo D, Zhang J. Integrative transcriptomic and metabonomic profiling analyses reveal the molecular mechanism of Chinese traditional medicine huankuile suspension on TNBS-induced ulcerative colitis. Aging. 2021;13(4):5087–103.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Yuan Z, Yang L, Zhang X, Ji P, Hua Y, Wei Y. Mechanism of Huang-lian-Jie-du decoction and its effective fraction in alleviating acute ulcerative colitis in mice: regulating arachidonic acid metabolism and glycerophospholipid metabolism. J Ethnopharmacol. 2020;259: 112872.

    Article  CAS  PubMed  Google Scholar 

  35. Wang D, Ma X, Guo S, Wang Y, Li T, Zou D, et al. Effect of Huangqin Tang on urine metabolic profile in rats with ulcerative colitis based on UPLC-Q-exactive orbitrap MS. Evid Based Complement Alternat Med. 2020;2020:1874065.

    PubMed  PubMed Central  Google Scholar 

  36. Wang D, Shi K, Wang Y, Zou D, Guo S, Li T, et al. Effect of Huangqin Tang on colonic gene expression in rats with ulcerative colitis. Int J Genom. 2020;2020:4238757.

    Google Scholar 

  37. Luo S, Wen R, Wang Q, Zhao Z, Nong F, Fu Y, et al. Rhubarb Peony decoction ameliorates ulcerative colitis in mice by regulating gut microbiota to restoring Th17/Treg balance. J Ethnopharmacol. 2019;231:39–49.

    Article  CAS  PubMed  Google Scholar 

  38. Amagase K, Kimura Y, Wada A, Yukishige T, Murakami T, Nakamura E, et al. Prophylactic effect of monosodium glutamate on NSAID-induced enteropathy in rats. Curr Pharm Des. 2014;20(16):2783–90.

    Article  CAS  PubMed  Google Scholar 

  39. Chen F, Yin YT, Zhao HM, Wang HY, Zhong YB, Long J, et al. Sishen pill treatment of DSS-induced colitis via regulating interaction with inflammatory dendritic cells and gut microbiota. Front Physiol. 2020;11:801.

    Article  PubMed  PubMed Central  Google Scholar 

  40. Ma H, Tao W, Zhu S. T lymphocytes in the intestinal mucosa: defense and tolerance. Cell Mol Immunol. 2019;16(3):216–24.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  41. Zhong YB, Kang ZP, Zhou BG, Wang HY, Long J, Zhou W, et al. Curcumin regulated the homeostasis of memory T cell and ameliorated dextran sulfate sodium-induced experimental colitis. Front Pharmacol. 2020;11: 630244.

    Article  CAS  PubMed  Google Scholar 

  42. Wu B, Wan Y. Molecular control of pathogenic Th17 cells in autoimmune diseases. Int Immunopharmacol. 2020;80: 106187.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  43. Tanoue T, Atarashi K, Honda K. Development and maintenance of intestinal regulatory T cells. Nat Rev Immunol. 2016;16(5):295–309.

    Article  CAS  PubMed  Google Scholar 

  44. Haase S, Haghikia A, Wilck N, Muller DN, Linker RA. Impacts of microbiome metabolites on immune regulation and autoimmunity. Immunology. 2018;154(2):230–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  45. Zhao Y, Luan H, Jiang H, Xu Y, Wu X, Zhang Y, et al. Gegen Qinlian decoction relieved DSS-induced ulcerative colitis in mice by modulating Th17/Treg cell homeostasis via suppressing IL-6/JAK2/STAT3 signaling. Phytomedicine. 2021;84: 153519.

    Article  CAS  PubMed  Google Scholar 

  46. Zhang M, Fan H, Tan S, Tang Q, Liu X, Zuo D, et al. The Chinese medicinal herb decoction QRZSLXF enhances anti-inflammatory effect in TNBS-induced colitis via balancing Th17/Tregs differentiation. J Ethnopharmacol. 2020;251: 112549.

    Article  CAS  PubMed  Google Scholar 

  47. Xu M, Duan XY, Chen QY, Fan H, Hong ZC, Deng SJ, et al. Effect of compound sophorae decoction on dextran sodium sulfate (DSS)-induced colitis in mice by regulating Th17/Treg cell balance. Biomed Pharmacother. 2019;109:2396–408.

    Article  CAS  PubMed  Google Scholar 

  48. Wen J, Teng B, Yang P, Chen X, Li C, Jing Y, et al. The potential mechanism of Bawei Xileisan in the treatment of dextran sulfate sodium-induced ulcerative colitis in mice. J Ethnopharmacol. 2016;188:31–8.

    Article  PubMed  Google Scholar 

  49. Liu XK, Zhao HM, Wang HY, Ge W, Zhong YB, Long J, et al. Regulatory effect of Sishen pill on Tfh cells in mice with experimental colitis. Front Physiol. 2020;11:589.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  50. Waldner MJ, Neurath MF. Mechanisms of immune signaling in colitis-associated cancer. Cell Mol Gastroenterol Hepatol. 2015;1(1):6–16.

    Article  PubMed  Google Scholar 

  51. Perkins ND. Post-translational modifications regulating the activity and function of the nuclear factor kappa B pathway. Oncogene. 2006;25(51):6717–30.

    Article  CAS  PubMed  Google Scholar 

  52. Li F, Zhang J, Arfuso F, Chinnathambi A, Zayed ME, Alharbi SA, et al. NF-kappaB in cancer therapy. Arch Toxicol. 2015;89(5):711–31.

    Article  CAS  PubMed  Google Scholar 

  53. He Z, Zhou Q, Wen K, Wu B, Sun X, Wang X, et al. Huangkui Lianchang decoction ameliorates DSS-induced ulcerative colitis in mice by inhibiting the NF-kappaB signaling pathway. Evid Based Complement Alternat Med. 2019;2019:1040847.

    PubMed  PubMed Central  Google Scholar 

  54. Chen YL, Zheng YY, Dai YC, Zhang YL, Tang ZP. Systems pharmacology approach reveals protective mechanisms of Jian-Pi Qing-Chang decoction on ulcerative colitis. World J Gastroenterol. 2019;25(21):2603–22.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  55. Hu Z, Yang M, Ye Q, Qin K, Wu M, Gu R, et al. Tou Nong San attenuates inflammation in TNBS-IBD model by inhibiting NF-kappaB signaling pathway. Evid Based Complement Alternat Med. 2018;2018:6929307.

    PubMed  PubMed Central  Google Scholar 

  56. Zheng L, Zhang YL, Dai YC, Chen X, Chen DL, Dai YT, et al. Jianpi Qingchang decoction alleviates ulcerative colitis by inhibiting nuclear factor-kappaB activation. World J Gastroenterol. 2017;23(7):1180–8.

    Article  PubMed  PubMed Central  Google Scholar 

  57. Dai YC, Zheng L, Zhang YL, Chen X, Chen DL, Wang LJ, et al. Jianpi Qingchang decoction regulates intestinal motility of dextran sulfate sodium-induced colitis through reducing autophagy of interstitial cells of Cajal. World J Gastroenterol. 2017;23(26):4724–34.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  58. Candia E, Diaz-Jimenez D, Langjahr P, Nunez LE, de la Fuente M, Farfan N, et al. Increased production of soluble TLR2 by lamina propria mononuclear cells from ulcerative colitis patients. Immunobiology. 2012;217(6):634–42.

    Article  CAS  PubMed  Google Scholar 

  59. Frolova L, Drastich P, Rossmann P, Klimesova K, Tlaskalova-Hogenova H. Expression of Toll-like receptor 2 (TLR2), TLR4, and CD14 in biopsy samples of patients with inflammatory bowel diseases: upregulated expression of TLR2 in terminal ileum of patients with ulcerative colitis. J Histochem Cytochem. 2008;56(3):267–74.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  60. Li R, Chen Y, Shi M, Xu X, Zhao Y, Wu X, et al. Gegen Qinlian decoction alleviates experimental colitis via suppressing TLR4/NF-kappaB signaling and enhancing antioxidant effect. Phytomedicine. 2016;23(10):1012–20.

    Article  PubMed  Google Scholar 

  61. Liu B, Piao X, Niu W, Zhang Q, Ma C, Wu T, et al. Kuijieyuan decoction improved intestinal barrier injury of ulcerative colitis by affecting TLR4-dependent PI3K/AKT/NF-kappaB oxidative and inflammatory signaling and gut microbiota. Front Pharmacol. 2020;11:1036.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  62. Zhou HY, Zhu H, Yao XM, Qian JP, Yang J, Pan XD, et al. Metformin regulates tight junction of intestinal epithelial cells via MLCK-MLC signaling pathway. Eur Rev Med Pharmacol Sci. 2017;21(22):5239–46.

    PubMed  Google Scholar 

  63. Xiong Y, Wang C, Shi L, Wang L, Zhou Z, Chen D, et al. Myosin light chain kinase: a potential target for treatment of inflammatory diseases. Front Pharmacol. 2017;8:292.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  64. Kang X, Jia M, Zhao L, Zhang S. Bu-Zhong-Yi-Qi granule enhances colonic tight junction integrity via TLR4/NF-kappaB/MLCK signaling pathway in ulcerative colitis rats. Evid Based Complement Alternat Med. 2021;2021:6657141.

    PubMed  PubMed Central  Google Scholar 

  65. Chao L, Li Z, Zhou J, Chen W, Li Y, Lv W, et al. Shen-Ling-Bai-Zhu-San improves dextran sodium sulfate-induced colitis by inhibiting caspase-1/caspase-11-mediated pyroptosis. Front Pharmacol. 2020;11:814.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  66. Wieacker P, Zimmer J, Ropers HH. X inactivation patterns in two syndromes with probable X-linked dominant, male lethal inheritance. Clin Genet. 1985;28(3):238–42.

    Article  CAS  PubMed  Google Scholar 

  67. Brott BK, Pinsky BA, Erikson RL. Nlk is a murine protein kinase related to Erk/MAP kinases and localized in the nucleus. Proc Natl Acad Sci USA. 1998;95(3):963–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  68. Ota S, Ishitani S, Shimizu N, Matsumoto K, Itoh M, Ishitani T. NLK positively regulates Wnt/beta-catenin signalling by phosphorylating LEF1 in neural progenitor cells. EMBO J. 2012;31(8):1904–15.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  69. Wang HY, Zhao HM, Wang Y, Liu Y, Lu XY, Liu XK, et al. Sishen Wan((R)) ameliorated trinitrobenzene-sulfonic-acid-induced chronic colitis via NEMO/NLK signaling pathway. Front Pharmacol. 2019;10:170.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  70. Li S, Wu Y, Yang D, Wu C, Ma C, Liu X, et al. Gasdermin D in peripheral myeloid cells drives neuroinflammation in experimental autoimmune encephalomyelitis. J Exp Med. 2019;216(11):2562–81.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  71. Zhao W, He F, Barkema HW, Xu S, Gao J, Liu G, et al. Prototheca spp. induce an inflammatory response via mtROS-mediated activation of NF-kappaB and NLRP3 inflammasome pathways in bovine mammary epithelial cell cultures. Vet Res. 2021;52(1):144.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  72. Qiao H, Huang Y, Chen X, Yang L, Wang Y, Xu R. Jiaweishaoyao decoction alleviates DSS-induced ulcerative colitis via inhibiting inflammation. Gastroenterol Res Pract. 2020;2020:7182874.

    Article  PubMed  PubMed Central  Google Scholar 

  73. Zhang Z, Shen P, Xie W, Cao H, Liu J, Cao Y, et al. Pingwei San ameliorates dextran sulfate sodium-induced chronic colitis in mice. J Ethnopharmacol. 2019;236:91–9.

    Article  CAS  PubMed  Google Scholar 

  74. Alkhadar H, Macluskey M, White S, Ellis I. Nerve growth factor-induced migration in oral and salivary gland tumour cells utilises the PI3K/Akt signalling pathway: is there a link to perineural invasion? J Oral Pathol Med. 2020;49(3):227–34.

    Article  CAS  PubMed  Google Scholar 

  75. Gabbouj S, Ryhanen S, Marttinen M, Wittrahm R, Takalo M, Kemppainen S, et al. Altered insulin signaling in Alzheimer’s disease brain—special emphasis on PI3K-Akt pathway. Front Neurosci. 2019;13:629.

    Article  PubMed  PubMed Central  Google Scholar 

  76. Zhou BG, Liu FC, Zhao HM, Zhang XY, Wang HY, Liu DY. Regulatory effect of Zuojin pill on correlation with gut microbiota and Treg cells in DSS-induced colitis. J Ethnopharmacol. 2020;262: 113211.

    Article  CAS  PubMed  Google Scholar 

  77. Ge W, Wang HY, Zhao HM, Liu XK, Zhong YB, Long J, et al. Effect of Sishen pill on memory T cells from experimental colitis induced by dextran sulfate sodium. Front Pharmacol. 2020;11:908.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  78. Wang B, Gong Z, Zhan J, Yang L, Zhou Q, Yuan X. Xianglian pill suppresses inflammation and protects intestinal epithelial barrier by promoting autophagy in DSS induced ulcerative colitis mice. Front Pharmacol. 2020;11: 594847.

    Article  CAS  PubMed  Google Scholar 

  79. Li MY, Luo HJ, Wu X, Liu YH, Gan YX, Xu N, et al. Anti-inflammatory effects of Huangqin decoction on dextran sulfate sodium-induced ulcerative colitis in mice through regulation of the gut microbiota and suppression of the Ras-PI3K-Akt-HIF-1alpha and NF-kappaB pathways. Front Pharmacol. 2019;10:1552.

    Article  CAS  PubMed  Google Scholar 

  80. Leonard WJ, O’Shea JJ. Jaks and STATs: biological implications. Annu Rev Immunol. 1998;16:293–322.

    Article  CAS  PubMed  Google Scholar 

  81. Hiller J, Hagl B, Effner R, Puel A, Schaller M, Mascher B, et al. STAT1 gain-of-function and dominant negative STAT3 mutations impair IL-17 and IL-22 immunity associated with CMC. J Invest Dermatol. 2018;138(3):711–4.

    Article  CAS  PubMed  Google Scholar 

  82. Li X, Wang Y, Wang K, Wu Y. Renal protective effect of paeoniflorin by inhibition of JAK2/STAT3 signaling pathway in diabetic mice. Biosci Trends. 2018;12(2):168–76.

    Article  CAS  PubMed  Google Scholar 

  83. Lu Z, Xiong W, Xiao S, Lin Y, Yu K, Yue G, et al. Huanglian Jiedu decoction ameliorates DSS-induced colitis in mice via the JAK2/STAT3 signalling pathway. Chin Med. 2020;15:45.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  84. Li L, Shen A, Chu J, Sferra TJ, Sankararaman S, Ke X, et al. Pien Tze Huang ameliorates DSS-induced colonic inflammation in a mouse colitis model through inhibition of the IL-6/STAT3 pathway. Mol Med Rep. 2018;18(1):1113–9.

    CAS  PubMed  Google Scholar 

  85. Xuan-Qing C, Xiang-Yu LV, Shi-Jia LIU. Baitouweng decoction alleviates dextran sulfate sodium-induced ulcerative colitis by regulating intestinal microbiota and the IL-6/STAT3 signaling pathway. J Ethnopharmacol. 2021;265: 113357.

    Article  CAS  PubMed  Google Scholar 

  86. Foldi J, Chung AY, Xu H, Zhu J, Outtz HH, Kitajewski J, et al. Autoamplification of Notch signaling in macrophages by TLR-induced and RBP-J-dependent induction of Jagged1. J Immunol. 2010;185(9):5023–31.

    Article  CAS  PubMed  Google Scholar 

  87. Bray SJ. Notch signalling: a simple pathway becomes complex. Nat Rev Mol Cell Biol. 2006;7(9):678–89.

    Article  CAS  PubMed  Google Scholar 

  88. Zhao Y, Luan H, Gao H, Wu X, Zhang Y, Li R. Gegen Qinlian decoction maintains colonic mucosal homeostasis in acute/chronic ulcerative colitis via bidirectionally modulating dysregulated Notch signaling. Phytomedicine. 2020;68: 153182.

    Article  CAS  PubMed  Google Scholar 

  89. Wu H, Chen QY, Wang WZ, Chu S, Liu XX, Liu YJ, et al. Compound sophorae decoction enhances intestinal barrier function of dextran sodium sulfate induced colitis via regulating notch signaling pathway in mice. Biomed Pharmacother. 2021;133: 110937.

    Article  CAS  PubMed  Google Scholar 

  90. Lea SR, Reynolds SL, Kaur M, Simpson KD, Hall SR, Hessel EM, et al. The effects of repeated Toll-like receptors 2 and 4 stimulation in COPD alveolar macrophages. Int J Chron Obstruct Pulmon Dis. 2018;13:771–80.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  91. Balka KR, de Nardo D. Understanding early TLR signaling through the Myddosome. J Leukoc Biol. 2019;105(2):339–51.

    Article  CAS  PubMed  Google Scholar 

  92. Wang S, Guo C, Zhang T, Zhong C, Zhao X, Su Y, et al. Tiaochang Xiaoyan extract tablets ameliorate chronic inflammation by activating macrophage lysosomes in chronic colitis rats. Ann Palliat Med. 2021;10(2):2203–16.

    Article  PubMed  Google Scholar 

  93. Zarling JM, Shoyab M, Marquardt H, Hanson MB, Lioubin MN, Todaro GJ. Oncostatin M: a growth regulator produced by differentiated histiocytic lymphoma cells. Proc Natl Acad Sci USA. 1986;83(24):9739–43.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  94. Verstockt S, Verstockt B, Machiels K, Vancamelbeke M, Ferrante M, Cleynen I, et al. Oncostatin M is a biomarker of diagnosis, worse disease prognosis, and therapeutic nonresponse in inflammatory bowel disease. Inflamm Bowel Dis. 2021;27(10):1564–75.

    Article  PubMed  PubMed Central  Google Scholar 

  95. Li Y, Chen F, Xie Y, Yang Q, Luo H, Jia P, et al. Feiyangchangweiyan capsule protects against ulcerative colitis in mice by modulating the OSM/OSMR pathway and improving gut microbiota. Phytomedicine. 2021;80: 153372.

    Article  CAS  PubMed  Google Scholar 

  96. Corr M. Wnt signaling in ankylosing spondylitis. Clin Rheumatol. 2014;33(6):759–62.

    Article  PubMed  Google Scholar 

  97. Sharma M, Castro-Piedras I, Simmons GE Jr, Pruitt K. Dishevelled: a masterful conductor of complex Wnt signals. Cell Signal. 2018;47:52–64.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  98. Zhu H, Li YR. Oxidative stress and redox signaling mechanisms of inflammatory bowel disease: updated experimental and clinical evidence. Exp Biol Med. 2012;237(5):474–80.

    Article  CAS  Google Scholar 

  99. Wu ZC, Zhao ZL, Deng JP, Huang JT, Wang YF, Wang ZP. Sanhuang Shu’ai decoction alleviates DSS-induced ulcerative colitis via regulation of gut microbiota, inflammatory mediators and cytokines. Biomed Pharmacother. 2020;125: 109934.

    Article  CAS  PubMed  Google Scholar 

  100. Gao H, Li Y, Sun J, Xu H, Wang M, Zuo X, et al. Saccharomyces boulardii ameliorates dextran sulfate sodium-induced ulcerative colitis in mice by regulating NF-kappaB and Nrf2 signaling pathways. Oxid Med Cell Longev. 2021;2021:1622375.

    PubMed  PubMed Central  Google Scholar 

  101. Li J, Ichikawa T, Janicki JS, Cui T. Targeting the Nrf2 pathway against cardiovascular disease. Expert Opin Ther Targets. 2009;13(7):785–94.

    Article  CAS  PubMed  Google Scholar 

  102. He M, Pan H, Chang RC, So KF, Brecha NC, Pu M. Activation of the Nrf2/HO-1 antioxidant pathway contributes to the protective effects of Lycium barbarum polysaccharides in the rodent retina after ischemia-reperfusion-induced damage. PLoS ONE. 2014;9(1): e84800.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  103. Zhang J, Kang X, Sun M, Zhang S. Qingre Jianpi decoction attenuates inflammatory responses by suppressing NOD-like receptor family pyrin domain-containing 3 inflammasome activation in dextran sulfate sodium-induced colitis mice. J Tradit Chin Med. 2021;41(1):68–78.

    PubMed  Google Scholar 

  104. Yu T, Li Z, Xu L, Yang M, Zhou X. Anti-inflammation effect of Qingchang suppository in ulcerative colitis through JAK2/STAT3 signaling pathway in vitro and in vivo. J Ethnopharmacol. 2021;266: 113442.

    Article  CAS  PubMed  Google Scholar 

  105. Shao J, Liu Z, Wang L, Song Z, Chang H, Han N, et al. Screening of the optimized prescription from Suqingwan in terms of its therapeutic effect on DSS-induced ulcerative colitis by its regulation of inflammatory and oxidative mediators. J Ethnopharmacol. 2017;202:54–62.

    Article  PubMed  Google Scholar 

  106. Liu B, Liu T, Wang X, Zheng X, Wang H, Ma L. Effects of Guchang capsule on dextran sulphate sodium-induced experimental ulcerative colitis in mice. Evid Based Complement Alternat Med. 2016;2016:3150651.

    PubMed  PubMed Central  Google Scholar 

  107. Wang R, Wu G, Du L, Shao J, Liu F, Yang Z, et al. Semi-bionic extraction of compound turmeric protects against dextran sulfate sodium-induced acute enteritis in rats. J Ethnopharmacol. 2016;190:288–300.

    Article  CAS  PubMed  Google Scholar 

  108. Wang X, Fan F, Cao Q. Modified Pulsatilla decoction attenuates oxazolone-induced colitis in mice through suppression of inflammation and epithelial barrier disruption. Mol Med Rep. 2016;14(2):1173–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  109. Chen YL, Zhang YL, Dai YC, Tang ZP. Systems pharmacology approach reveals the antiinflammatory effects of Ampelopsis grossedentata on dextran sodium sulfate-induced colitis. World J Gastroenterol. 2018;24(13):1398–409.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  110. Zhang W, Chao X, Wu JQ, Ma XB, Yang YL, Wu Y, et al. Exploring the potential mechanism of Guchang Zhixie Wan for treating ulcerative colitis by comprehensive network pharmacological approaches and molecular docking validation as well as cell experiments. Chem Biodivers. 2021;18(1): e2000810.

    CAS  PubMed  Google Scholar 

  111. Jimbo K, Ohtsuka Y, Kojima Y, Hosoi K, Ohbayashi N, Ikuse T, et al. Increased expression of CXCR3 axis components and matrix metalloproteinase in pediatric inflammatory bowel disease patients. Pediatr Int. 2014;56(6):873–83.

    Article  CAS  PubMed  Google Scholar 

  112. Liu M, Guo S, Hibbert JM, Jain V, Singh N, Wilson NO, et al. CXCL10/IP-10 in infectious diseases pathogenesis and potential therapeutic implications. Cytokine Growth Factor Rev. 2011;22(3):121–30.

    CAS  PubMed  PubMed Central  Google Scholar 

  113. Mao TY, Shi R, Zhao WH, Guo Y, Gao KL, Chen C, et al. Qingchang Wenzhong decoction ameliorates dextran sulphate sodium-induced ulcerative colitis in rats by downregulating the IP10/CXCR3 axis-mediated inflammatory response. Evid Based Complement Alternat Med. 2016;2016:4312538.

    PubMed  PubMed Central  Google Scholar 

  114. Kreida S, Roche JV, Olsson C, Linse S, Tornroth-Horsefield S. Protein–protein interactions in AQP regulation—biophysical characterization of AQP0-CaM and AQP2-LIP5 complex formation. Faraday Discuss. 2018;209:35–54.

    Article  CAS  PubMed  Google Scholar 

  115. Benga G. Water channel proteins (later called aquaporins) and relatives: past, present, and future. IUBMB Life. 2009;61(2):112–33.

    Article  CAS  PubMed  Google Scholar 

  116. Pan X, Xu K, Xiong R, Chen ZM, Hu CJ, Feng J, et al. Therapeutic effect of Ershen pill () extract on Pi (spleen)-Shen (kidney) yang deficiency-induced diarrhea in rat model. Chin J Integr Med. 2019;25(7):536–42.

    Article  PubMed  Google Scholar 

  117. Sun B, Yuan J, Wang S, Lin J, Zhang W, Shao J, et al. Qingchang suppository ameliorates colonic vascular permeability in dextran-sulfate-sodium-induced colitis. Front Pharmacol. 2018;9:1235.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  118. Wang X, Li D, Zhang Y, Wu S, Tang F. Costus root granules improve ulcerative colitis through regulation of TGF-beta mediation of the PI3K/AKT signaling pathway. Exp Ther Med. 2018;15(5):4477–84.

    PubMed  PubMed Central  Google Scholar 

  119. Wu M, Shen A, Chen Y, Liu L, Li L, Sankararaman S, et al. Xinhuang tablets improve intestinal barrier function via regulating epithelial tight junctions in dextran sulfate sodium-induced ulcerative colitis mice. J Med Food. 2021;24(1):33–9.

    Article  CAS  PubMed  Google Scholar 

  120. Ding Y, Chen M, Wang Q, Gao L, Feng Y, Wang S, et al. Integrating pharmacology and microbial network analysis with experimental validation to reveal the mechanism of composite sophora colon-soluble capsule against ulcerative colitis. Evid Based Complement Alternat Med. 2020;2020:9521073.

    PubMed  PubMed Central  Google Scholar 

  121. Wang W, Xu C, Li X, Wang Z, Yang J, Shen Y, et al. Exploration of the potential mechanism of Banxia Xiexin decoction for the effects on TNBS-induced ulcerative colitis rats with the assistance of network pharmacology analysis. J Ethnopharmacol. 2021;277: 114197.

    Article  CAS  PubMed  Google Scholar 

  122. Wei Y, Jiang N, Liu T, Liu C, Xiao W, Liang L, et al. The comparison of extraction methods of ganjiang decoction based on fingerprint, quantitative analysis and pharmacodynamics. Chin Med. 2020;15:81.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  123. Buchner AM, Schneider Y, Lichtenstein GR. Biosimilars in inflammatory bowel disease. Am J Gastroenterol. 2021;116(1):45–56.

    Article  PubMed  Google Scholar 

  124. Zhu HH, Hu J, Lo-Coco F, Jin J. The simpler, the better: oral arsenic for acute promyelocytic leukemia. Blood. 2019;134(7):597–605.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

The study was supported by the Center for Chronic Disease Research at Dalian University College of Medicine and Yanbian University College of Pharmacy.

Funding

This work was supported by funds from the Korean Food Research Project (2017029) and the National Natural Science Foundation of China (61671098).

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Author notes

  1. Qing-Gao Zhang

    Present address: Chronic Disease Research Center, Medical College, Dalian University, Dalian, 116622, Liaoning, China

Authors and Affiliations

  1. Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji, 133002, Jilin, China

    Shuo Yuan, Ji-Xing Nan & Qing-Gao Zhang

  2. Chronic Disease Research Center, Medical College, Dalian University, Dalian, 116622, Liaoning, China

    Shuo Yuan, Qi Wang, Jiao Li, Jia-Chen Xue, You Li, Huan Meng & Xiao-Ting Hou

  3. Department of Immunology and Pathogenic Biology, Yanbian University College of Basic Medicine, Yanji, 133002, Jilin, China

    Jiao Li, Jia-Chen Xue & Qing-Gao Zhang

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  1. Shuo Yuan
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Contributions

SY wrote the manuscript. QW collected the literature and wrote part of the manuscript. JL and J-CX collected the literature. YL, HM, and X-TH revised the manuscript. J-XN and Q-GZ presented the research ideas and revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ji-Xing Nan or Qing-Gao Zhang.

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Yuan, S., Wang, Q., Li, J. et al. Inflammatory bowel disease: an overview of Chinese herbal medicine formula-based treatment. Chin Med 17, 74 (2022). https://doi.org/10.1186/s13020-022-00633-4

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Keywords

Chinese Medicine

ISSN: 1749-8546

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